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A laboratory guide to the mammalian embryo

Author: David K Gardner; Michelle Lane; Andrew J Watson
Publisher: Oxford ; New York : Oxford University Press, 2004.
Edition/Format:   Print book : EnglishView all editions and formats
Database:WorldCat
Summary:

Offers a range of research techniques - from cell culture, to biochemical, to microscopic and genetic - to study the early mammalian embryo. This book is aimed at a range of researchers, from basic  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Manuels de laboratoire
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: David K Gardner; Michelle Lane; Andrew J Watson
ISBN: 0195171519 9780195171518 0195142268 9780195142266
OCLC Number: 51461213
Description: xiv, 394 pages : illustrations (some color) ; 29 cm
Contents: Follicle development in vitro / Norah Spears, Evelyn E. Telfer and Alison A. Murray --
Preparation of gametes, in vitro maturation, in vitro fertilization, and embryo recovery and transfer / Michelle Lane and David K. Gardner --
Culture of the mammalian preimplantation embryo / David K. Gardner and Michelle Lane --
Assessment of preimplanation embryo development and viability / Denny Sakkas, Michelle Lane and David K. Gardner --
Micromanipulation of gametes / Gianpiero D. Palermo [and others] --
Micromanipulation: biopsy / Joyce C. Harper and Alpesh Doshi --
Analysis of intracellular ions in embryos: pH and calcium / Michelle Lane, Jay M. Baltz, and David K. Gardner --
Assessment of nutrient uptake, metabolite production, and enzyme activity / David K. Gardner and Michelle Lane --
Metabolic pathway activity / Don Rieger --
Confocal imaging of structural molecules in mammalian gametes / Calvin Simerly [and others]. Fluorescent in situ hybridization for dection of aneuploidy in single human blastomeres for preimplanation genetic diagnosis / Leeanda Wilton --
Assessment of ploidy, telomere length, and telomerase activity in oocytes and embryos / W. Allan King [and others] --
Microscale RNA isolation from mammalian embryos / C. Wrenzycki, Andrew J. Watson, and Michele Calder --
Relative mRNA transcript abundance in early embryos by reverse transcription-polymerase chain reaction / Paul A. de Sousa and Andrew J. Watson. Characterization of novel genes during early development by application of differential display RT-PCR / David R. Nataleand Andrew J. Watson --
Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells / Keith E. Latham [and others]. Antisense disruption of gene expression in the preimplantation embryo / Lisa C. Barcroft, D. Holstead Jones-Taggart and Andrew J. Watson --
Gene subtraction and analysis / Marc-André and Claude Robert --
Analysis of apoptosis in the preimplantation embryo / Daniel R. Brison [and others] --
Cytoplasmic signaling and cell cycle control in the mouse egg and embryo / D. Page Baluch, Christine M. Pauken, and David G. Capco --
Human embryonic stem cells / Susan E. Lazendorf, Catherine A. Boyd, and Diane L. Wright --
Spermatogonial transplantion / Makoto Nagano --
Somatic cell nuclear transfer / Paul de Sousa [and others] --
Cryobiology: slow freezing vitrification of embryos / Tetsunori Mukaida and Magosaburo Kasai.
Responsibility: edited by David K. Gardner, Michelle Lane, Andrew J. Watson.
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