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A laboratory guide to the mammalian embryo

Author: David K Gardner; Michelle Lane; Andrew J Watson
Publisher: Oxford ; New York : Oxford University Press, 2004.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as "Manipulating the Mouse Embryo", from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to  Read more...
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Genre/Form: Laboratory Manuals
Electronic books
Laboratory manuals
Manuels de laboratoire
Additional Physical Format: Print version:
Laboratory guide to the mammalian embryo.
Oxford ; New York : Oxford University Press, 2004
(DLC) 2003000329
(OCoLC)51461213
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: David K Gardner; Michelle Lane; Andrew J Watson
ISBN: 9780198032519 019803251X 0195302737 9780195302738 0195171519 9780195171518 0195142268 9780195142266 1429415177 9781429415170 1602567875 9781602567870 1280427884 9781280427886 1280481722 9781280481727
OCLC Number: 60314677
Description: 1 online resource (xiv, 394 pages) : illustrations (some color)
Contents: Follicle development in vitro / Norah Spears, Evelyn E. Telfer and Alison A. Murray --
Preparation of gametes, in vitro maturation, in vitro fertilization, and embryo recovery and transfer / Michelle Lane and David K. Gardner --
Culture of the mammalian preimplantation embryo / David K. Gardner and Michelle Lane --
Assessment of preimplanation embryo development and viability / Denny Sakkas, Michelle Lane and David K. Gardner --
Micromanipulation of gametes / Gianpiero D. Palermo [and others] --
Micromanipulation: biopsy / Joyce C. Harper and Alpesh Doshi --
Analysis of intracellular ions in embryos: pH and calcium / Michelle Lane, Jay M. Baltz, and David K. Gardner --
Assessment of nutrient uptake, metabolite production, and enzyme activity / David K. Gardner and Michelle Lane --
Metabolic pathway activity / Don Rieger --
Confocal imaging of structural molecules in mammalian gametes / Calvin Simerly [and others]. Fluorescent in situ hybridization for dection of aneuploidy in single human blastomeres for preimplanation genetic diagnosis / Leeanda Wilton --
Assessment of ploidy, telomere length, and telomerase activity in oocytes and embryos / W. Allan King [and others] --
Microscale RNA isolation from mammalian embryos / C. Wrenzycki, Andrew J. Watson, and Michele Calder --
Relative mRNA transcript abundance in early embryos by reverse transcription-polymerase chain reaction / Paul A. de Sousa and Andrew J. Watson. Characterization of novel genes during early development by application of differential display RT-PCR / David R. Nataleand Andrew J. Watson --
Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells / Keith E. Latham [and others]. Antisense disruption of gene expression in the preimplantation embryo / Lisa C. Barcroft, D. Holstead Jones-Taggart and Andrew J. Watson --
Gene subtraction and analysis / Marc-André and Claude Robert --
Analysis of apoptosis in the preimplantation embryo / Daniel R. Brison [and others] --
Cytoplasmic signaling and cell cycle control in the mouse egg and embryo / D. Page Baluch, Christine M. Pauken, and David G. Capco --
Human embryonic stem cells / Susan E. Lazendorf, Catherine A. Boyd, and Diane L. Wright --
Spermatogonial transplantion / Makoto Nagano --
Somatic cell nuclear transfer / Paul de Sousa [and others] --
Cryobiology: slow freezing vitrification of embryos / Tetsunori Mukaida and Magosaburo Kasai.
Responsibility: edited by David K. Gardner, Michelle Lane, Andrew J. Watson.
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Abstract:

A compendium that offers a range of research techniques-from cell culture, to biochemical, to microscopic and genetic. This book is aimed at a broad range of researchers, from basic experimental  Read more...

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