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Laboratory methods for the detection of mutations and polymorphisms in DNA

Author: G R Taylor
Publisher: Boca Raton : CRC Press, ©1997.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Deals with the experimental analysis of DNA and presents various protocols. This book details various techniques from the simple to the highly complex. It is suitable for researchers working in  Read more...

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Genre/Form: Laboratory Manuals
Laboratory manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: G R Taylor
ISBN: 0849392330 9780849392337
OCLC Number: 34912808
Description: 333 pages : illustrations ; 26 cm
Contents: Arbitrarily Primed PCR Fingerprints/F. Mathieu-Daude, D. Ralph, and M. McClelland --
Minisatellite Analysis/G. Rysiecki, W.P. Childs, and N. Duxbury --
Microsatellite Analysis/S. Payne --
ARMS Analysis of Point Mutations/S. Little --
Solid-Phase Minisequencing/A.-C. Syvanen --
Mutation Detection using Oligonucleotide Ligation/E. S. Winn-Deen --
Combined Single Strand Conformation Polymorphism and heteroduplex Analysis/A. J. Wallace --
Denaturing Gradient Gel Electrophoreses/M. Losekoot and R. Fodde --
Coupled Transcription-Translation/T. Hamzehleoi and S. West --
Peptide Nucleic Acids/H. Orum, T. Koch, M. Egholm, H. O'Keefe, and J. Coull --
Orphan Peak Analysis/M. Hattori, H. Hagiwara, and Y. Sakaki --
Sequence-Specific Gene Signatures Produced by Low Stringency Single Specific Polymerase Chain Reaction/A. J.G. Simpson and S.D.J. Pena --
Bisulphite Genomic Sequencing of Methylated Cytosines/S. J. Clar and M. Frommer --
Direct Sequencing of Polymerase Chain Reaction Products/E. Werle --
Heterozygote Determination Using Automated DNA Sequencing Technology/M. N. Kronick --
A Robust Radioactive Sequencing Protocol for Double-Stranded Polymerase Chain Reaction Products/M. D. Robinson and K.J. Leach --
Use of MutY and Thymine Glycosylase to Detect Point Mutations/I.-C. Hsu --
MutS-Exonuclease Protection for the Detection of Mismatched Heteroduplexes/V. H. Laitinen, G.R. Taylor, S. Hamilton, and P. Flick --
Enzyme Mismatch Cleavage-Bacteriophage Resolvases/R. D. Mashal and J. Sklar --
Chemical Cleavage of Mismatch in Heteroduplexes/J. Prosser --
Comparison of Mutation Detection by Chemical Cleavage of Mismatch adn Enzyme Mismatch Cleavage/R. Youil, S.J. Ramus, and R.G.H. Cotton --
Analysis of DNA Sequence Using Biosensors/P. Nilsson, B. Persson, M. Uhlen, and P.-A. Nygren --
Repeat Expansion Detection/C. Zander, C.E. Burgess, J. Johansen, K. Lindblad, T. Hudson, and M. Schalling --
Comparative Genomic Hybridization/A. Kallioniemi, R. Karhu, and O.-P. Kallioniemi --
Large-Scale Electrophoretic Studies: Microplate Array Diagonal Gel Electrophoresis (MADGE) and Profiling of Oligonucleotide Dissociation by Gel Electrophoresis (PODGE)/I. N.M. Day, S.D. O'Dell, G.P. Weavind, and S.E. Humphries --
DNA Sequence Database Searches, Comparisons and Primer Design/G. Williams --
Accessing Genome Data: The Human Genome Database, Internet and WWW/G. Williams.
Responsibility: edited by Graham R. Taylor.
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