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Laboratory Techniques in Biochemistry and Molecular Biology, 18 : Methods of Cell Separation.

Author: P T Sharpe
Publisher: Burlington : Elsevier, 1988.
Series: Laboratory techniques in biochemistry and molecular biology.
Edition/Format:   eBook : Document : English
Summary:
Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific  Read more...
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Details

Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: P T Sharpe
ISBN: 9780080858876 0080858872
OCLC Number: 476219351
Description: 1 online resource (287 pages).
Contents: Front Cover; Methods of Cell Separation; Copyright Page; Abbreviations; Contents; Chapter 1. Introduction; Chapter 2. Methods of cell counting and assaying cell viability; Chapter 3. Centrifugation; Chapter 4. Unit gravity sedimentation; Chapter 5. Centrifugal elutriation; Chapter 6. Aqueous two-phase partition; Chapter 7. Electrophoresis; Specific surface methods --
an introduction to Chapters 8-13; Chapter 8. Affinity chromatography; Chapter 9. Affinity separation on fibres and surfaces; Chapter 10. Affinity partition; Chapter 11. Flow sorting; Chapter 12. Magnetic cell sorting.
Series Title: Laboratory techniques in biochemistry and molecular biology.

Abstract:

Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Me.

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