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The LINC complex : methods and protocols

Author: Gregg G Gundersen; Howard J Worman
Publisher: New York, NY : Humana Press, 2018.
Series: Methods in molecular biology (Clifton, N.J.), v. 1840.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in  Read more...

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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: (OCoLC)1034605003
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Gregg G Gundersen; Howard J Worman
ISBN: 9781493986910 1493986910
OCLC Number: 1049975224
Description: 1 online resource (xiv, 338 pages) : illustrations (some color).
Contents: EM tomography of meiotic LINC complexes / Marie-Christin Spindler, Frederik Helmprobst, Christian Stigloher, and Ricardo Benavente --
Recombinant purification of the periplasmic portion of the LINC complex / Victor E. Cruz and Thomas U. Schwartz --
Analysis of high molecular weight isoforms of nesprin-1 and nesprin-2 with vertical agarose gel electrophoresis / Chloe Potter and Didier Hodzic --
Analysis of nesprin-2 interaction with its binding partners and actin / Susumu Antoku and Gregg G. Gundersen --
Interactions of nesprin-4-containing LINC complexes in outer hair cells explored by BioID / Brian Burke --
Using nesprin tension sensors to measure force on the LINC complex / Paul T. Arsenovic and Daniel E. Conway --
Analyzing mechanotransduction through the LINC complex in isolated nuclei / Nejma Belaadi, Angélique Millon-Frémillon, Julien Aureille, and Christophe Guilluy --
Direct force probe for nuclear mechanics / Vincent J. Tocco, Srujana Neelam, Qiao Zhang, Richard B. Dickinson, and Tanmay P. Lele --
Centrifugal displacement of nuclei in adherent cells to study LINC complex-dependent mechanisms of homeostatic nuclear positioning / Ruijun Zhu and Gregg G. Gundersen --
Assembly and use of a microfluidic device to study cell migration in confined environments / Jeremy Keys, Aaron Windsor, and Jan Lammerding --
Investigating LINC complex protein homo-oligomerization in the nuclear envelopes of living cells using fluorescence fluctuation spectroscopy / Jared Hennen, Isaac Angert, Kwang-Ho Hur, G. W. Gant Luxton, and Joachim D. Mueller --
Functional analysis of the yeast LINC complex using fluctuation spectroscopy and super-resolution imaging / Jay R. Unruh, Brian D. Slaughter, and Sue L. Jaspersen --
Genetic analysis of nuclear migration and anchorage to study LINC complexes during development of Caenorhabditis elegans / Heidi N. Fridolfsson, Leslie A. Herrera, James N. Brandt, Natalie E. Cain, Greg J. Hermann, and Daniel A. Starr --
High-resolution imaging methods to analyze LINC complex function during Drosophila muscle eevelopment / Alexander L. Auld, Mary Ann Collins, Torrey R. Mandigo, and Eric S. Folker --
Computational methods for studying the plant nucleus / Axel Poulet, Xiao Zhou, Kentaro Tamura, Iris Meier, Christophe Tatout, Katja Graumann, and David E. Evans --
Investigation of nuclear periphery protein interactions in plants using the membrane yeast two-hybrid (MbY2H) system / Maxime Voisin, Emmanuel Vanrobays, and Christophe Tatout --
Immunolabeling protocols for studying meiosis in plant mutants defective for nuclear envelope components / Javier Varas and Mónica Pradillo --
Generation and analysis of striated muscle selective LINC complex protein mutant mice / Matthew J. Stroud, Xi Fang, Jennifer Veevers, and Ju Chen --
In vitro system to measure the positioning, stiffness, and rupture of the nucleus in skeletal muscle / William Roman, Mafalda R. Pimentel, and Edgar R. Gomes --
Functional analysis of LINC complexes in the skin / Iakowos Karakesisoglou, Carmen Mross, and Angelika A. Noegel --
Detection of SUN1 splicing variants at the mRNA and protein levels in cancer / Ayaka Matsumoto, Nariaki Matsuura, and Miki Hieda --
Next-generation sequencing and mutational analysis : implications for genes encoding LINC complex proteins / Peter L. Nagy and Howard J. Worman.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1840.
Responsibility: edited by Gregg G. Gundersen, Howard J. Worman.

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