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Macrophages and dendritic cells : methods and protocols

Author: Neil E Reiner
Publisher: New York : Humana, ©2009.
Series: Methods in molecular biology (Clifton, N.J.), v. 531.; Springer protocols (Series)
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and  Read more...
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Genre/Form: Laboratory Manuals
Electronic books
Additional Physical Format: Print version:
Macrophages and dendritic cells.
New York : Humana, ©2009
(DLC) 2008942272
(OCoLC)262719462
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Neil E Reiner
ISBN: 9781588299727 1588299724 9781597453967 159745396X
OCLC Number: 319004963
Language Note: English.
Description: 1 online resource (xii, 368 pages) : illustrations (some color).
Contents: Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors / Georgia Schäfer and Gordon D. Brown --
Generation of murine growth factor-dependent long-term dendritic cell lines to investigate host-parasite interactions / Alessandra Mortellaro [and others] --
Macrophage activation : classical vs. alternative / Andrea Classen, Jorge Lloberas, and Antonio Celada --
Analysis of macrophage phagocytosis : quantitative assays of phagosome formation and maturation using high-throughput florescence microscopy / Benjamin E. Steinberg and Sergio Grinstein --
Detection of activated Rab7 GTPase with an immobilized RILP probe / Jim Sun [and others] --
Probing in vivo origins of mononuclear phagocytes by conditional ablation and reconstitution / Chen Varol, Limor Landsman, and Steffen Jung --
Multivalent protein probes for the identification and characterization of cognate cellular ligands for myeloid cell surface receptors / Hsi-Hsien Lin [and others] --
Exploiting Fc chimaeric proteins for the identification of ligands specific for the mannose receptor / Luisa Martinez-Pomares --
The expression of exogenous genes in macrophages : obstacles and opportunities / Xia Zhang, Justin P. Edwards, and David M. Mosser --
Genetic modification of murine dendritic cells by RNA transfection / Maurizio Ceppi, Enrico Schmidt, and Philippe Pierre --
Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities / Robin M. Yates, Albin Hermetter, and David G. Russell --
Derivation and characterization of murine alternatively activated (M2) macrophages / Victor W.H. Ho and Laura M. Sly --
Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology / Mattias Svensson --
Generation, culture, and flow-cytometric characterization of primary mouse macrophages / Ulrike Schleicher and Christian Bogdan --
Analyzing classical and alternative macrophage activation in macrophage/neutrophil-specific IL-4 receptor-alpha-deficient mice / Frank Brombacher [and others] --
Analysis of matrix metalloproteinase secretion by macrophages / Paul T. Elkington, Justin A. Green, and Jon S. Friedland --
Identification of pathogen receptors on dendritic cells to understand their function and to identify new drug targets / Lot de Witte [and others] --
Stable lentiviral vector-mediated gene silencing in human monocytic cell lines / Jimmy S. Lee and Neil E. Reiner --
Use of fluorescent probes to detect lipid signaling intermediates in macrophages / Vivan Kwok, Eric Vachon, and Gregory P. Downey --
Large-scale phagosome preparation / Adrien F. Vinet and Albert Descoteaux --
Coordinate regulation of sphingosine kinase and actin dynamics / Shankar S. Iyer and David J. Kusner.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 531.; Springer protocols (Series)
Responsibility: edited by Neil E. Reiner.

Abstract:

Expert researchers contribute laboratory protocols involving these two vital cell types at the junction of the innate and acquired immune systems. The volume looks at isolation and cell culturing and  Read more...

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