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Making and using antibodies : a practical handbook

Author: Gary C Howard; Matthew R Kaser
Publisher: Boca Raton, FL : Taylor & Francis/CRC Press, ©2014.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:
"This second edition provides guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present descriptions of basic methods for making and using  Read more...
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Genre/Form: Laboratory Manuals
Laboratory manuals
Document Type: Book
All Authors / Contributors: Gary C Howard; Matthew R Kaser
ISBN: 9781439869086 1439869081
OCLC Number: 802325678
Description: xiv, 444 pages : illustrations (some color) ; 24 cm
Contents: Antibodies; Matthew R. Kaser and Gary C. HowardAntigens and Adjuvants; Paul Algate, Jory Baldridge, and Sally P. MossmanProduction of Polyclonal Antibodies; Lon V. KendallPurification and Characterization of Antibodies; Joseph P. ChandlerProduction of Monoclonal Antibodies; Kathleen C. F. SheehanQuantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. SmithMaking Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. SidhuIn Vitro Selection of Aptamers; Tianjiao Wang, Xiangyu Cong, and Marit Nilsen-HamiltonChemical and Proteolytic Modification of Antibodies; George P. SmithWestern Blots and Other Applications; Lee Bendickson and Marit Nilsen-HamiltonImmunohistochemical Methods; Jose A. Ramos-VaraImmunoelectron Microscopy; Sara E. Miller and David N. HowellFlow Cytometry; Steven B. McClellanELISAs; John Chen and Gary C. HowardHumanization of Antibodies; Juan Carlos Almagro, Sreekumar Kodangattil, and Jian LiAntibodies in the Future: Challenges and Opportunities; Gary C. Howard and Matthew R. KaserIndex
Responsibility: edited by Gary C. Howard, Matthew R. Kaser.

Abstract:

"This second edition provides guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Each chapter includes introductory material, allowing for a better understanding of each concept. Practical examples are also included"--Provided by publisher.

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