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Malaria methods and protocols

Author: Denise L Doolan
Publisher: Totowa, N.J. : Humana Press, ©2002.
Series: Methods in molecular medicine, 72.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Despite considerable scientific and medical effort over the past decades, malaria remains the most important human parasitic disease. It is responsible for up to 3 million deaths and another 300-500 million new cases each year, and is becoming resistant to the current chemoprophylactic and chemotherapeutic agents. In Malaria Methods and Protocols, internationally respected scientists and clinicians describe in  Read more...
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Genre/Form: Fulltext
Illustration
Index not Present
Internet Resource
Laboratory Manual
Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Malaria methods and protocols.
Totowa, N.J. : Humana Press, ©2002
(DLC) 2001051656
(OCoLC)48162854
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Denise L Doolan
ISBN: 0896038238 9780896038233 9781592592715 1592592716 1280830565 9781280830563 9786610830565 6610830568
OCLC Number: 170845101
Language Note: English.
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xv, 631 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Vector incrimination and entomological inoculation rates --
Epidemiological measures of risk of malaria --
Maintenance of the Plasmodium berghei life cycle --
Mouse models for pre-erythrocytic-stage malaria --
Mouse models for erythrocytic-stage malaria --
Nonhuman primate models. I. Nonhuman primate host-parasite combinations --
Nonhuman primate models. II. Infection of Saimiri and Aotus monkeys with Plasmodium vivax --
Vector analysis --
Genotyping of Plasmodium spp. Nested PCR --
Genotyping of Plasmodium falciparum. PCR-RFLP analysis --
Microsatellite analysis in Plasmodium falciparum --
Quantitation of liver-stage parasites by automated TaqMan real-time PCR --
Quantitation of liver-stage parasites by competitive RT-PCR --
Extraction and purification of Plasmodium total RNA --
Extraction and purification of Plasmodium parasite DNA --
Southern blotting of parasite DNA --
SDS-PAGE and western blotting of Plasmodium falciparum proteins --
Nested PCR analysis of Plasmodium parasites --
RFLP analysis --
Analysis of gene expression by RT-PCR --
In situ detection of RNA in blood- and mosquito-stage malaria parasites --
Purification of chromosomes from Plasmodium falciparum --
Construction of genomic libraries from the DNA of Plasmodium species --
Construction of a gene library with mung bean nuclease-treated genomic DNA --
Construction of Plasmodium falciparum lambda cDNA libraries --
Production of stage-specific Plasmodium falciparum cDNA libraries using subtractive hybridization --
Construction and screening of YAC libraries --
Episomal transformation of Plasmodium berghei --
Gene targeting in Plasmodium berghei --
Peptide vaccination --
DNA vaccination --
Assessing antigen-specific CD8+ and CD4+ T-cell responses in mice after immunization with recombinant viruses --
Assessing CD4+ helper T-lymphocyte responses by lymphoproliferation --
Limiting dilution analysis of antigen-specific CD4+ T-cell responses in mice --
Cell trafficking. Malaria blood-stage parasite-specific CD4+ T cells after adoptive transfer into mice --
Assessing antigen-specific proliferation and cytokine responses using flow cytometry --
Cytokine analysis by intracellular staining --
Assessment of antigen-specific CTL- and CD8(+)-dependent IFN-gamma responses in mice --
Assessing antigen-specific CD8+ CTL responses in humans --
Human antibody subclass ELISA --
Systemic nitric oxide production in human malaria. I. Analysis of NO metabolites in biological fluids --
Systemic nitric oxide production in human malaria. II. Analysis of mononuclear cell nitric oxide synthase type 2 antigen expression --
In vitro culture of Plasmodium parasites --
Automated synchronization of Plasmodium falciparum parasites by culture in a temperature-cycling incubator --
Hepatic portal branch inoculation --
Hepatocyte perfusion, isolation, and culture --
Inhibition of sporozoite invasion. The double-staining assay --
Inhibition of liver-stage development assay --
T-cell mediated inhibition of liver-stage development assay --
Antibody-dependent cellular inhibition assay --
Erythrocytic malaria growth or invasion inhibition assays with emphasis on suspension culture GIA --
Analysis of CSA-binding parasites and antiadhesion antibodies --
Analysis of the adhesive properties of Plasmodium falciparum-infected red blood cells under conditions of flow --
Preparation of adhesive targets for flow-based cytoadhesion assays --
Triton X-114 phase partitioning for antigen characterization --
Immunoprecipitation for antigen localization --
Field trials.
Series Title: Methods in molecular medicine, 72.
Responsibility: edited by Denise L. Doolan.
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Abstract:

Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed  Read more...

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"In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria." - Clinical Read more...

 
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