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Mammalian oocyte regulation : methods and protocols

Author: Hayden A Homer
Publisher: New York : Humana Press : Springer, ©2013.
Series: Methods in molecular biology (Clifton, N.J.), v. 957.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This Methods in Molecular Biology (TM) volume offers methodologies enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte  Read more...

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Genre/Form: Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Hayden A Homer
ISBN: 9781627031905 1627031901
OCLC Number: 802321698
Description: xi, 311 pages : illustrations (some color) ; 26 cm.
Contents: Studying recombination in mouse oocytes / Xianfei Sun and Paula E. Cohen --
Analysis of recombinants in female mouse meiosis / Esther de Boer, Maria Jasin, and Scott Keeney --
Studying meiosis-specific cohesins in mouse embryonic oocytes / Ji-hye Kim [and others] --
Immunohistochemical approaches to the study of human fetal ovarian development / Jing He [and others] --
Protein kinase assays for measuring MPF and MAPK activities in mouse and rat oocytes and early embryos / Jacek Z. Kubiak --
Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes / Janet E. Holt, Simon I.R. Lane, and Keith T. Jones --
Using FRET to study ranGTP gradients in live mouse oocytes / Julien Dumont and Marie-Hélène Verlhac --
Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes / Mark Levasseur --
RNAi-based methods for gene silencing in mouse oocytes / Paula Stein, Petr Svoboda, and Richard M. Schultz --
Micro-injection of morpholino oligonucleotides for depleting securin in mouse oocytes / Petros Marangos --
Measuring transport and accumulation of radiolabeled substrates in oocytes and embryos / Jay M. Baltz, Hannah E. Corbett, and Samantha Richard --
Immunofluorescence staining of spindles, chromosomes, and kinetochores in human oocytes / Solon Riris [and others] --
Studying the roles of aurora-C kinase during meiosis in mouse oocytes / Kuo-Tai Yang [and others] --
Chromosome spreads with centromere staining in mouse oocytes / Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann --
Preparation of mammalian oocytes for transmission electron microscopy / Lynne Anguish and Scott Coonrod --
Measuring Ca² oscillations in mammalian eggs / Karl Swann --
Counting chromosomes in intact eggs / Teresa Chiang and Michael A. Lampson --
Free-hand bisection of mouse oocytes and embryos / Zbigniew Polanski and Jacek Z. Kubiak --
Microarray-CGH for the assessment of aneuploidy in human polar bodies and oocytes / Souraya Jaroudi and Dagan Wells --
Nuclear transfer in the mouse oocyte / Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 957.; Springer protocols (Series)
Responsibility: edited by Hayden A. Homer.

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