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The Mammalian Preimplantation Embryo : Regulation of Growth and Differentiation in Vitro

Author: Barry D Bavister
Publisher: Boston, MA : Springer US, 1987.
Edition/Format:   eBook : Bibliographic data : EnglishView all editions and formats
Summary:

With a few notable exceptions, mammalian preimplantation embryos grown in vitro are likely to exhibit sub-optimal or retarded development.

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Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Bibliographic data, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Barry D Bavister
ISBN: 9781468453324 1468453327 9781468453348 1468453343
OCLC Number: 840289079
Description: 1 online resource (398 pages)
Contents: 1 Pioneering Mammalian Embryo Culture --
1. Introduction --
2. Early Period Using Media Prepared From Biological Fluids --
3. Successful Culture of a Rodent Preimplantation Embryo --
4. Culture of Rabbit Embryos Revisited --
5. Conclusion --
6. References --
2 Cell Polarity in the Preimplantation Mouse Embryo --
1. Introduction --
2. The Unfertilized Egg --
3. The Fertilized Egg --
4. 2-Cell and 4-Cell Stages --
5. The 8-Cell Stage Embryo --
6. Developmental Significance of Embryonic Polarities --
7. References --
3 Intercellular Communication during Mouse Embryogenesis --
1. Introduction --
2. Communication Via Cytoplasmic Bridges --
3. Communication Via Intercellular Membrane Channels --
4. Communication Via Cell Surface Interaction --
5. On the Role of Intercellular Communication Pathways in Embryogenesis --
6. References --
4 Development of the Blastocyst: Role of Cell Polarity in Cavitation and Cell Differentiation --
1. Introduction --
2. Three Models for Cavitation: Cell Polarity and the Production of Nascent Blastocoele Fluid --
3. Electrical Polarity of Outer Blastomeres from Mouse Morulae --
4. Summary --
5. References --
5 In Vitro Assessment of Blastocyst Differentiation --
1. Introduction --
Overview of Blastogenesis --
2. Oxidative Metabolism --
3. Trans-Trophectodermal Sodium Transport --
4. Protein Synthesis During Blastogenesis --
5. Characterization of Blastocyst Plasma Membranes --
6. Conclusions: Future Perspectives --
7. References --
6 Steroid Hormones in Early Pig Embryo Development --
1. Introduction --
2. Methodology --
3. Progesterone: Embryonic Uptake and Its Specificity --
4. In Vitro Culture of Morulae in the Presence of Supplementary Steroids --
5. Estradiol-17 Beta: Embryonic Uptake and Its Specificity --
6. Estradiol Withdrawal and In Vitro Development of Morula Stages --
7. Further Considerations --
8. References --
7 Genetic Expression during Early Mouse Development --
1. Introduction --
2. Synthesis of Maternally-Derived Products --
3. Activation of the Embryonic Genome --
4. Are Maternally-Derived Products Important for Development Beyond the 2-Cell Stage? --
5. Both the Maternal and Paternal Genomes Are Required for Normal Development --
6. Is It Possible To Clone Mammals by Nuclear Transfer? --
7. Mutations and Chromosomal Abnormalities That Affect the Preimplantation Mouse Embryo --
8. Genetic Control of Early Mammalian Development: Future Approaches --
9. Summary --
10. References --
8 Defining the Roles of Growth Factors during Early Mammalian Development --
1. Introduction --
2. Preimplantation Mouse Embryos Cultured In Serum-Supplemented Media --
3. Mouse Embryos Cultured in Serum-Free Media --
4. Design of Serum-Free Media for Mouse Blastocysts --
5. Production of Growth Factors by Early Embryos --
6. Possible Roles of Growth Factors During Early Postimplantation Development --
7. Possible Effects of Growth Factors on the Process of Differentiation --
8. Conclusions --
9. Appendix --
10. References --
9 Interaction of Trophoblastic Vesicles with Bovine Embryos Developing in Vitro --
1. Introduction --
2. Working With Trophoblastic Vesicles --
3. Effect of Trophoblastic Vesicles on Early Stage Embryo Development In Vitro --
4. Evidence for Release by Trophoblastic Vesicles of Signals For Corpus Luteum Function --
5. Conclusions --
6. References --
10 In Vitro Growth of Preimplantation Rabbit Embryos --
1. Introduction --
2. Relevance of Rabbit Embryo Culture to Studies of Embryonic Development --
3. How Does Growth of Cultured Rabbit Embryos Compare With Growth In Vivo? --
4. In Vitro Growth Requirements of Rabbit Embryos --
5. Methodological Considerations --
6. Evaluation of Embryos --
7. Conclusions --
8. References --
11 Studies on the Developmental Blocks in Cultured Hamster Embryos --
1. Introduction --
2. Technical Procedures --
3. The 2-Cell Block to Development --
4. Growth of 8-Cell Embryos In Vitro --
5. Conclusions --
6. References --
12 Growth of Domesticated Animal Embryos in Vitro --
1. Introduction --
2. Media, Supplements and Antibiotics --
3. Glucose Metabolism During Embryo Development --
4. Embryo Co-Culture --
5. References --
13 In Vitro Growth of Non-Human Primate Pre- and Peri- Implantation Embryos --
1. Introduction --
2. Technical Considerations --
3. Supply of In Vivo Fertilized Embryos --
4. In Vitro Fertilized Embryos --
5. Embryo Growth Characteristics --
6. Assessment of Embryonic Normalcy after In Vitro Culture --
7. Conclusions --
8. Appendix: Culture Media --
9. References --
14 Analysis of Embryotoxic Effects in Preimplantation Embryos --
1. Introduction --
2. Sensitive Toxicological Endpoints --
3. Exposure of Embryos In Vitro --
4. Exposure of Embryos In Vivo --
5. Conclusions --
6. References --
15 Applications of Animal Embryo Culture Research to Human IVF and Embryo Transfer Programs --
1. Introduction --
2. Studies on Animal IVF, Embryo Culture and Transfer --
3. Present Status and Prospects of Human IVF-ET --
4. Conclusions --
5. References --
Appendix I --
1. Introduction --
2. Conditions for Embryo Culture --
3. Equipment --
4. Experimental Design --
5. Evaluation of Responses to Embryo Culture --
6. Blocks to Development --
7. References --
Appendix II --
1. Supplies for Embryo Culture Experiments --
2. Equipment --
3. Manufacturers and Distributors of Supplies and Equipment.
Responsibility: edited by Barry D. Bavister.

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