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Manipulating the mouse embryo : a laboratory manual

Author: Brigid Hogan
Publisher: Plainview, N.Y. : Cold Spring Harbor Laboratory Press, 1994.
Edition/Format:   Print book : English : 2nd edView all editions and formats
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Genre/Form: Laboratory manuals
Laboratory Manual
Manuels de laboratoire
Document Type: Book
All Authors / Contributors: Brigid Hogan
ISBN: 0879693843 9780879693848
OCLC Number: 30783431
Description: xvii, 497 pages : illustrations (some color) ; 29 cm
Contents: Developmental genetics and embryology of the mouse: past, present and future --
Summary of mouse development --
Setting up a colony for the production of transgenic and mutant mice --
Recovery, culture and transfer of embryos and germ cells --
In vitro manipulation of preimplantation embryos --
Production of transgenic mice --
Isolation, culture and manipulation of embryonic stem cells --
Analysis of transgenic mice --
Techniques for visualizing genes, gene products and specialized cell types. In vitro culture of eggs, embryos, primordial germ cells and teratocarcinoma cells --
Buffers and solutions --
Acidic tyrode solution for removing zonae --
Alkaline phosphatase buffers --
Alkaline phosphatase staining solution --
Avertin, anesthetic --
Bovine serum albumin (BSA) --
Ca/Mg⁺⁺-free PBS --
Ca⁺⁺/Mg⁺⁺-free tyrode ringer's saline (pH 7.6-7.7) --
Hyaluronidase --
NTM buffer (NTMT without tween 20) for in situ hybridization of intact embryos with RNA probes --
NTMT buffer for in situ hybridization of intact embryos with RNA probes. Pancreatin/trypsin solution for separating germ and tissue layers --
PBSMT for immunohistochemistry of whole mount embryos --
PBT --
Phenol/chloroform solution for isolating total RNA from mouse embryos or fetal tissues --
Phosphate-buffered saline (PBS ̲--
Pronase solution --
Saline/EDTA buffer plus glucose for isolation of germ cells and tissue culture --
20X SSC --
20X SSPE --
TE (tris/EDTA) buffer --
0.25% trypsin in tris-saline for tissue culture --
Trypsin/EDTA solutions --
Sources of information --
Genetic resources --
Books --
Journals. Databases --
Software --
Suppliers --
Inbred, hybrid and random-bred mice --
Mouse genomic DNAs and RNAs --
Mouse genomic and cDNA libraries and clones --
Equipment and supplies.
Responsibility: Brigid Hogan [and others].

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