跳至内容
Manipulation and expression of recombinant DNA : a laboratory manual 線上預覽
關閉線上預覽
正在查...

Manipulation and expression of recombinant DNA : a laboratory manual

作者: Dominique Robertson; Scott H Shore; David M Miller
出版商: San Diego : Academic Press, ©1997.
版本/格式:   Print book : 英語所有版本和格式的總覽
資料庫:WorldCat
提要:
(Publisher-supplied data) This laboratory manual will be an indispensable tool for students, by providing in depth descriptions of protocols which cover the basic aspects of molecular biology and biochemistry. The book covers techniques in detail, and teaches students to culture and transform bacteria, grow vector plasmids, purify insert DNA, ligate vector and insert, and ultimately express protein and assay its  再讀一些...
評定級別:

(尚未評分) 0 附有評論 - 成爲第一個。

主題
更多類似這樣的

 

在圖書館查詢

&AllPage.SpinnerRetrieving; 正在查詢有此資料的圖書館...

詳細書目

類型/形式: Laboratory Manuals
Laboratory manuals
資料類型: 網際網路資源
文件類型: 圖書, 網路資源
所有的作者/貢獻者: Dominique Robertson; Scott H Shore; David M Miller
ISBN: 0125897650 9780125897655
OCLC系統控制編碼: 37770587
描述: xix, 204 pages : illustrations ; 28 cm
内容: Part 1 Manipulation of DNA: Lab session 1 - culturing E. Coli for plasmid isolation: preparation of solid media, making stabs cultures, streaking out plasmid clones on LB/carb plates, growth curve of E. Coli,; cultures for plasmid preparations; Lab session 2 - growth curve of E. Coli: examination of clones, E. Coli growth curve, preparation of solutions for large scale plasmid DNA preps, inoculation of cultures for large scale plasmid DNA preps; Lab session 3A - large scale preparation of plasmid DNA by alkaline lysis; Lab session 3B - purification of plasmid DNA using columns; Lab session 3C - purification of plasmid DNA using cesium gradients; Lab session 3D - purification of plasmid DNA using polyethylene glycol; Lab session 4 - preparation of vector DNA - ethidium bromide spot test, preparation of Hindill Cut, phosphated vector, gel electrophoresis, electrophoresis of pUR288, shrimp alkaline phosphatase treatment of pUR288, overnight cultures for transformation-competent cells; Lab session 5 - transformation of E. Coli - preparation of frozen, transformation-competent cells, test ligations of pUR288 electrophoresis of ligation products, transformation; Lab session 6 - preparation and ligation of insert DNA: analysis of vector preparation, isolation of myo-3 DNA from agarose, ligations; Lab session 7A - transformation of E. Coli with ligated DNA and labelling a DNA probe: CaCl2-mediated transformation, electrophoration, electrophoresis of ligation reactions, random primed DNA labelling with Dig-11-dUTP; Lab session 7B: labelling DNA with radioactive nucleotides: random primer labelling of DNA using 32p-dCTP; Lab Session 8 - DNA probe preparation, replica plating: estimating the yield of the DIG-labelled probe, making replica plates; Lab session 9 - colony hybrid dilations, DNA probe: colony hybridizations - transfer of colonies to nylon membranes, prehybridization, DNA/DNA hybridization, adding DNA probe; Lab session 10 - restriction of map of plasmid p2D, colony nybridizations. Part 2 Restriction enzyme reactions; colony hybridization with a DNA probe - washing filters probed with Dig-11-dUTP labelled myo-3 fragment; colony hybridization with a DNA probe - washing filters probed with 32p-dCTP labelled myo-3 fragment; gel electrophoresis of restriction digests; Lab session 11 - screening colonies with monoclonal antibodies: colony hybridizations - mAb probe, first day, alkaline lysis minipreps, finish restriction maps; Lab session 12 - isolation and characterization of miniprep DNA from potential transformants. (Part contents).
責任: Dominique Robertson, Scott Shore, David M. Miller.
更多資訊:

摘要:

This laboratory manual is designed for use in introductory biotechnology courses. Its main objective is to expose students to the basic research protocols used in molecular biology and biochemistry  再讀一些...

評論

社評

出版商概要

"This is a clearly written and well thought out laboratory manual that covers many of the basic concepts and laboratory techniques common to molecular biology... The book has many clear and concise 再讀一些...

 
讀者提供的評論
正在擷取GoodReads評論...
正在擷取DOGObooks的評論

標籤

成爲第一個
確認申請

你可能已經申請過這份資料。若還是想申請,請選確認。

連結資料


Primary Entity

<http://www.worldcat.org/oclc/37770587> # Manipulation and expression of recombinant DNA : a laboratory manual
    a schema:Book, schema:CreativeWork ;
   library:oclcnum "37770587" ;
   library:placeOfPublication <http://experiment.worldcat.org/entity/work/data/47466320#Place/san_diego> ; # San Diego
   library:placeOfPublication <http://id.loc.gov/vocabulary/countries/cau> ;
   schema:about <http://id.loc.gov/authorities/subjects/sh85053855> ; # Genetic engineering
   schema:about <http://experiment.worldcat.org/entity/work/data/47466320#Topic/genie_genetique_manuels_de_laboratoire> ; # Génie génétique--Manuels de laboratoire
   schema:about <http://experiment.worldcat.org/entity/work/data/47466320#Topic/genetic_engineering_laboratory_manuals> ; # Genetic engineering--Laboratory manuals
   schema:about <http://experiment.worldcat.org/entity/work/data/47466320#Topic/dna_recombinant> ; # DNA, Recombinant
   schema:about <http://id.worldcat.org/fast/940027> ; # Genetic engineering
   schema:about <http://experiment.worldcat.org/entity/work/data/47466320#Topic/adn_recombinant_manuels_de_laboratoire> ; # ADN recombinant--Manuels de laboratoire
   schema:about <http://experiment.worldcat.org/entity/work/data/47466320#Topic/protein_engineering> ; # Protein Engineering
   schema:about <http://id.loc.gov/authorities/subjects/sh85111851> ; # Recombinant DNA
   schema:about <http://experiment.worldcat.org/entity/work/data/47466320#Topic/adn_recombine_manuels_de_laboratoire> ; # ADN recombiné--Manuels de laboratoire
   schema:about <http://experiment.worldcat.org/entity/work/data/47466320#Topic/recombinant_dna_laboratory_manuals> ; # Recombinant DNA--Laboratory manuals
   schema:about <http://dewey.info/class/660.65/e21/> ;
   schema:about <http://id.worldcat.org/fast/1091466> ; # Recombinant DNA
   schema:bookFormat bgn:PrintBook ;
   schema:contributor <http://experiment.worldcat.org/entity/work/data/47466320#Person/miller_david_m> ; # David M. Miller
   schema:contributor <http://viaf.org/viaf/23716163> ; # Scott H. Shore
   schema:copyrightYear "1997" ;
   schema:creator <http://viaf.org/viaf/11460340> ; # Dominique Robertson
   schema:datePublished "1997" ;
   schema:description "(Publisher-supplied data) This laboratory manual will be an indispensable tool for students, by providing in depth descriptions of protocols which cover the basic aspects of molecular biology and biochemistry. The book covers techniques in detail, and teaches students to culture and transform bacteria, grow vector plasmids, purify insert DNA, ligate vector and insert, and ultimately express protein and assay its activity. Comprehensive and complete, this text covers the underlying techniques used in all basic research and biotechnology laboratories. Key Features Designed to cover the basic aspects of molecular biology and biochemistry. Features student-tested protocols. Presents experiments in a modular fashion, lending itself equally to the traditional semester course and the shorter intensive course. Covers important techniques and protocols used in all basic research labs. "Project" approach to experiments gives students an overview of entire process. Experiments incorporate immunological techniques that go beyond basic exercises in DNA cloning."@en ;
   schema:exampleOfWork <http://worldcat.org/entity/work/id/47466320> ;
   schema:genre "Laboratory manuals"@en ;
   schema:genre "Laboratory Manuals"@en ;
   schema:inLanguage "en" ;
   schema:name "Manipulation and expression of recombinant DNA : a laboratory manual"@en ;
   schema:productID "37770587" ;
   schema:publication <http://www.worldcat.org/title/-/oclc/37770587#PublicationEvent/san_diego_academic_press_1997> ;
   schema:publisher <http://experiment.worldcat.org/entity/work/data/47466320#Agent/academic_press> ; # Academic Press
   schema:url <http://catdir.loc.gov/catdir/toc/els032/97195982.html> ;
   schema:workExample <http://worldcat.org/isbn/9780125897655> ;
   umbel:isLike <http://bnb.data.bl.uk/id/resource/GB9763037> ;
   wdrs:describedby <http://www.worldcat.org/title/-/oclc/37770587> ;
    .


Related Entities

<http://experiment.worldcat.org/entity/work/data/47466320#Agent/academic_press> # Academic Press
    a bgn:Agent ;
   schema:name "Academic Press" ;
    .

<http://experiment.worldcat.org/entity/work/data/47466320#Person/miller_david_m> # David M. Miller
    a schema:Person ;
   schema:familyName "Miller" ;
   schema:givenName "David M." ;
   schema:name "David M. Miller" ;
    .

<http://experiment.worldcat.org/entity/work/data/47466320#Topic/adn_recombinant_manuels_de_laboratoire> # ADN recombinant--Manuels de laboratoire
    a schema:Intangible ;
   schema:name "ADN recombinant--Manuels de laboratoire"@fr ;
    .

<http://experiment.worldcat.org/entity/work/data/47466320#Topic/adn_recombine_manuels_de_laboratoire> # ADN recombiné--Manuels de laboratoire
    a schema:Intangible ;
   schema:name "ADN recombiné--Manuels de laboratoire"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/47466320#Topic/genetic_engineering_laboratory_manuals> # Genetic engineering--Laboratory manuals
    a schema:Intangible ;
   schema:name "Genetic engineering--Laboratory manuals"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/47466320#Topic/genie_genetique_manuels_de_laboratoire> # Génie génétique--Manuels de laboratoire
    a schema:Intangible ;
   schema:name "Génie génétique--Manuels de laboratoire"@en ;
   schema:name "Génie génétique--Manuels de laboratoire"@fr ;
    .

<http://experiment.worldcat.org/entity/work/data/47466320#Topic/protein_engineering> # Protein Engineering
    a schema:Intangible ;
   schema:name "Protein Engineering"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/47466320#Topic/recombinant_dna_laboratory_manuals> # Recombinant DNA--Laboratory manuals
    a schema:Intangible ;
   schema:name "Recombinant DNA--Laboratory manuals"@en ;
    .

<http://id.loc.gov/authorities/subjects/sh85053855> # Genetic engineering
    a schema:Intangible ;
   schema:name "Genetic engineering"@en ;
    .

<http://id.loc.gov/authorities/subjects/sh85111851> # Recombinant DNA
    a schema:Intangible ;
   schema:name "Recombinant DNA"@en ;
    .

<http://id.worldcat.org/fast/1091466> # Recombinant DNA
    a schema:Intangible ;
   schema:name "Recombinant DNA"@en ;
    .

<http://id.worldcat.org/fast/940027> # Genetic engineering
    a schema:Intangible ;
   schema:name "Genetic engineering"@en ;
    .

<http://viaf.org/viaf/11460340> # Dominique Robertson
    a schema:Person ;
   schema:birthDate "1951" ;
   schema:familyName "Robertson" ;
   schema:givenName "Dominique" ;
   schema:name "Dominique Robertson" ;
    .

<http://viaf.org/viaf/23716163> # Scott H. Shore
    a schema:Person ;
   schema:familyName "Shore" ;
   schema:givenName "Scott H." ;
   schema:name "Scott H. Shore" ;
    .

<http://worldcat.org/isbn/9780125897655>
    a schema:ProductModel ;
   schema:isbn "0125897650" ;
   schema:isbn "9780125897655" ;
    .


Content-negotiable representations

關閉視窗

請登入WorldCat 

没有帳號嗎?你可很容易的 建立免費的帳號.