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Manipulation and expression of recombinant DNA : a laboratory manual

Autor Susan Carson; Dominique Robertson
Vydavatel: Burlington, MA : Elsevier Academic Press, ©2006.
Vydání/formát:   book_printbook : English : 2nd edZobrazit všechny vydání a formáty
Databáze:WorldCat
Shrnutí:
Introduces advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.
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Detaily

Žánr/forma: Laboratory Manuals
Laboratory manuals
Manuels de laboratoire
Typ dokumentu: Book
Všichni autoři/tvůrci: Susan Carson; Dominique Robertson
ISBN: 0120884186 9780120884186
OCLC číslo: 63293147
Poznámky: Includes index.
Popis: xvi, 151 pages : illustrations (some color) ; 28 cm
Obsahy: Foreword Acknowledgements Note to Instructors Instrumentation Nomenclature INTRODUCTION: Conceptual outline for experiments PART I MANIPULATION OF DNA PART II SCREENING TRANSFORMANTS PART III EXPRESSION, DETECTION, AND PURIFICATION OF RECOMBINANT PROTEINS Appendices: Equipment, Prep list, Making sense of orientation
Odpovědnost: Susan Carson, Dominique Robertson.

Anotace:

Introduces the techniques of recombinant DNA technology, or gene cloning and expression. This work covers the techniques used in basic research and biotechnology laboratories. It includes exercises  Přečíst více...

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