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Manipulation and expression of recombinant DNA : a laboratory manual

Auteur : Susan Carson; Dominique Robertson
Éditeur: Burlington, MA : Elsevier Academic Press, ©2006.
Édition/format:   Livre imprimé : Anglais : 2nd edVoir toutes les éditions et tous les formats
Base de données:WorldCat
Résumé:
Introduces advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.
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Détails

Genre/forme: Laboratory manuals
Laboratory Manuals
Manuels de laboratoire
Type de document: Livre
Tous les auteurs / collaborateurs: Susan Carson; Dominique Robertson
ISBN: 0120884186 9780120884186
Numéro OCLC: 63293147
Notes: Includes index.
Description: xvi, 151 pages : illustrations (some color) ; 28 cm
Contenu: Conceptual outline for experiments --
Manipulation of DNA --
Screening transformats --
Expression, detection, and purification of recombinant proteins from bacteria --
Appendixes --
Index.
Responsabilité: Susan Carson, Dominique Robertson.

Résumé:

Introduces the techniques of recombinant DNA technology, or gene cloning and expression. This work covers the techniques used in basic research and biotechnology laboratories. It includes exercises  Lire la suite...

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