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Manipulation and expression of recombinant DNA : a laboratory manual

Autor: Susan Carson; Dominique Robertson
Editora: Burlington, MA : Elsevier Academic Press, ©2006.
Edição/Formato   Print book : Inglês : 2nd edVer todas as edições e formatos
Base de Dados:WorldCat
Resumo:
Introduces advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.
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Detalhes

Gênero/Forma: Laboratory Manuals
Laboratory manuals
Manuels de laboratoire
Tipo de Documento: Livro
Todos os Autores / Contribuintes: Susan Carson; Dominique Robertson
ISBN: 0120884186 9780120884186
Número OCLC: 63293147
Notas: Includes index.
Descrição: xvi, 151 pages : illustrations (some color) ; 28 cm
Conteúdos: Foreword Acknowledgements Note to Instructors Instrumentation Nomenclature INTRODUCTION: Conceptual outline for experiments PART I MANIPULATION OF DNA PART II SCREENING TRANSFORMANTS PART III EXPRESSION, DETECTION, AND PURIFICATION OF RECOMBINANT PROTEINS Appendices: Equipment, Prep list, Making sense of orientation
Responsabilidade: Susan Carson, Dominique Robertson.

Resumo:

Introduces the techniques of recombinant DNA technology, or gene cloning and expression. This work covers the techniques used in basic research and biotechnology laboratories. It includes exercises  Ler mais...

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