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Manipulation and expression of recombinant DNA : a laboratory manual

Autor: Susan Carson; Dominique Robertson
Editorial: Burlington, MA : Elsevier Academic Press, ©2006.
Edición/Formato:   Print book : Inglés (eng) : 2nd edVer todas las ediciones y todos los formatos
Base de datos:WorldCat
Resumen:
Introduces advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.
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Detalles

Género/Forma: Laboratory manuals
Laboratory Manuals
Manuels de laboratoire
Tipo de documento: Libro/Texto
Todos autores / colaboradores: Susan Carson; Dominique Robertson
ISBN: 0120884186 9780120884186
Número OCLC: 63293147
Notas: Includes index.
Descripción: xvi, 151 pages : illustrations (some color) ; 28 cm
Contenido: Foreword Acknowledgements Note to Instructors Instrumentation Nomenclature INTRODUCTION: Conceptual outline for experiments PART I MANIPULATION OF DNA PART II SCREENING TRANSFORMANTS PART III EXPRESSION, DETECTION, AND PURIFICATION OF RECOMBINANT PROTEINS Appendices: Equipment, Prep list, Making sense of orientation
Responsabilidad: Susan Carson, Dominique Robertson.

Resumen:

Introduces the techniques of recombinant DNA technology, or gene cloning and expression. This work covers the techniques used in basic research and biotechnology laboratories. It includes exercises  Leer más

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