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Manipulation and expression of recombinant DNA : a laboratory manual

Auteur : Susan Carson; Dominique Robertson
Éditeur : Burlington, MA : Elsevier Academic Press, ©2006.
Édition/format :   Livre imprimé : English : 2nd edVoir toutes les éditions et tous les formats
Base de données :WorldCat
Résumé :
Introduces advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.
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Détails

Genre/forme : Laboratory Manuals
Laboratory manuals
Manuels de laboratoire
Format : Book
Tous les auteurs / collaborateurs : Susan Carson; Dominique Robertson
ISBN : 0120884186 9780120884186
Numéro OCLC : 63293147
Notes : Includes index.
Description : xvi, 151 pages : illustrations (some color) ; 28 cm
Contenu : Foreword Acknowledgements Note to Instructors Instrumentation Nomenclature INTRODUCTION: Conceptual outline for experiments PART I MANIPULATION OF DNA PART II SCREENING TRANSFORMANTS PART III EXPRESSION, DETECTION, AND PURIFICATION OF RECOMBINANT PROTEINS Appendices: Equipment, Prep list, Making sense of orientation
Responsabilité : Susan Carson, Dominique Robertson.

Résumé :

Introduces the techniques of recombinant DNA technology, or gene cloning and expression. This work covers the techniques used in basic research and biotechnology laboratories. It includes exercises  Lire la suite...

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