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Mechanisms of DNA recombination and genome rearrangements. Methods to study homologous recombination

Author: Maria Spies; Anna Malkova
Publisher: Cambridge, MA : Academic Press, an imprint of Elsevier, 2018.
Series: Methods in enzymology, v. 600.
Edition/Format:   Print book : English : First editionView all editions and formats
Summary:
Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination, Volume 600, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity  Read more...
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Document Type: Book
All Authors / Contributors: Maria Spies; Anna Malkova
ISBN: 9780128144299 0128144297
OCLC Number: 1029874937
Description: xviii, 590 pages : illustrations (some color) ; 24 cm.
Contents: 1. Processing of DNA Double-Strand Breaks in Yeast / Robert Gnügge, Julyun Oh and Lorraine S. Symington --
2. Methods to Study DNA End Resection I: Recombinant Protein Purification / Roopesh Anand, Cosimo Pinto and Petr Cejka --
3. Methods to Study DNA End Resection II: Biochemical Reconstitution Assays / Cosimo Pinto, Roopesh Anand and Petr Cejka --
4. Direct Quantitative Monitoring of Homology-Directed DNA Repair of Damaged Telomeres / Priyanka Verma, Robert L. Dilley, Melina T. Gyparaki and Roger A. Greenberg --
5. Kinetic Analysis of the Exonuclease Activity of the Bacteriophage T4 Mre11-Rad50 Complex / Tibebe A. Teklemariam, Osvaldo D. Rivera and Scott W. Nelson --
6. Expression, Purification, and Biochemical Evaluation of Human RAD51 Protein / Shyamal Subramanyam and Maria Spies --
7. Determining the RAD51-DNA Nucleoprotein Filament Structure and Function By Cryo-Electron Microscopy / Lingyun Zhao, Jingfei Xu, Weixing Zhao, Patrick Sung, and Hong-Wei Wang --
8. Observation and Analysis of RAD51 Nucleation Dynamics t Sngle-Monomer Resolution / Shyamal Subramanyam, Colin D. Kinz-Thompson, Ruben L. Gonzalez Jr., and Maria Spies --
9. TIRF-Based Single-Molecule Detection of the RecA Presynaptic Filament Dynamics / Sung H. Kim --
10. The RadA Recombinase and Paralogs of the Hyperthermophilic Archaeon Sulfolobus Solfataricus / Michael L. Rolfsmeier and Cynthia A. Haseltine --
11. Reconstituting the 4-Strand DNA Strand Exchange / Olga M. Mazina and Alexander V. Mazin --
12. Purification Of Saccharomyces Cerevisiae Homologous Recombination Proteins Dmc1 and Rdh54/Tid1 and a Fluorescent D-Loop Assay / Yuen-Ling Chan and Douglas K. Bishop --
13. Probing Dynamic Assembly and Disassembly of Rad51 Tuned by Srs2 Using smFRET / Yupeng Qiu, Hye R. Koh and Sua Myong --
14. The Recombination Mediator BRCA2: Architectural Plasticity of Recombination Intermediates Revealed by Single-Molecule Imaging (SFM/TIRF) / Arshdeep Sidhu, Dejan Ristic, Humberto Sánchez and Claire Wyman --
15. Single-Molecule Dynamics and Localization of DNA Repair Proteins in Cells / Maarten W. Paul, Alex N. Zelensky, Claire Wyman and Roland Kanaar --
16. Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence Microscopy / Luisina De Tullio, Kyle Kaniecki and Eric C. Greene --
17. Single-Molecule Analysis of Replication Protein A-DNA Interactions / Fletcher E. Bain, Laura A. Fischer, Ran Chen and Marc S. Wold --
18. Single-Molecule Studies of ssDNA-Binding Proteins Exchange / Olivia Yang and Taekjip Ha --
19. Dissecting the Recombination Mediator Activity of BRCA2 Using Biochemical Methods / Catharina von Nicolai, Åsa Ehlén, Juan S. Martinez and Aura Carreira --
20. Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydis / Qingwen Zhou, William K. Holloman and Milorad Kojic --
21. GEN1 Endonuclease: Purification and Nuclease Assays / Ying Wai Chan and Stephen C. West --
22. Biochemical and Structural Properties of Fungal Holliday Junction-Resolving Enzymes / Yijin Liu, Alasdair Freeman, Anne-Cécile Déclais, Anton Gartner and David M. J. Lilley --
23. Preparation and Resolution of Holliday Junction DNA Recombination Intermediates / Rajvee Shah Punatar and Stephen C. West.
Series Title: Methods in enzymology, v. 600.
Other Titles: Methods to study homologous recombination
Responsibility: edited by Maria Spies, Anna Malkova.

Abstract:

Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination, Volume 600, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This volume, together with volume 601 (Mechanisms of DNA Recombination and Genome Rearrangements: Intersection between Homologous Recombination, DNA Replication and DNA Repair) offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination. The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts.

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