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Metastasis research protocols

Author: M V Dwek; Susan A Brooks; Udo Schumacher
Publisher: New York : Humana Press : Springer, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 878.; Springer protocols (Series)
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

Now in a second, revised edition, this practical companion to the methodologies of researching cancer growth includes topical introductions and stepwise, reproducible protocols for laboratory  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: M V Dwek; Susan A Brooks; Udo Schumacher
ISBN: 9781617798535 1617798533 9781617798542 1617798541
OCLC Number: 801937427
Description: xii, 274 pages : illustrations ; 27 cm.
Contents: Basic immunocytochemistry for light microscopy / Susan A. Brooks --
Lectin histochemistry to detect altered glycosylation in cells and tissues / Susan A. Brooks and Debbie M.S. Hall --
Histopathological assessment of metastasis / Derek E. Roskell and Ian D. Buley --
Immunocytochemical detection and characterisation of individual micrometastatic tumour cells / Stephan Braun and Klaus Pantel --
The polymerase chain reaction / Hazel M. Welch --
Sodium dodecyl sulphate-polyacrylamide denaturing gel electrophoresis and western blotting techniques / Christine Blancher and Rob McCormick --
2-DE-based proteomics for the analysis of metastasis-associated proteins / Miriam Dwek and Diluka Peiris --
Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography / Marta Toth, Anjum Sohail, and Rafael Fridman --
Determination of cell-specific receptor binding using a combination of immunohistochemistry and in vitro autoradiography : relevance to therapeutic receptor targeting in cancer / Michael R. Dashwood and Marilena Loizidou --
Fluorescence in situ hybridization for cancer-related studies / Lyndal Kearney and Janet Shipley --
Using a quartz crystal microbalance biosensor for the study of metastasis markers on intact cells / Julien Saint-Guirons and Björn Ingemarsson --
Cell separations by flow cytometry / Derek Davies --
Detection of putative cancer stem cells of the side population phenotype in human tumor cell cultures / Matthias Christgen [and others] --
Genes involved in the metastatic cascade of medullary thyroid tumours / Caroline Schreiber, Kirsten Vormbrock and Ulrike Ziebold --
High-resolution quantitative methylation analysis of microRNA genes using PyrosequencingTM / Ulrich Lehmann, Cord Albat, and Hans Kreipe --
RNAi technology to block the expression of molecules relevant to metastasis : the cell adhesion molecule CEACAM1 as an instructive example / Daniel Wicklein --
Galectin-3 binding and metastasis / Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz --
Lectin array-based strategies for identifying metastasis-associated changes in glycosylation / Simon Fry [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 878.; Springer protocols (Series)
Responsibility: edited by Miriam Dwek, Susan A. Brooks, Udo Schumacher.

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From the book reviews:"This second edition ... describes cellular and molecular techniques used to study cancer metastasis. ... It is written for any researchers interested in cellular and molecular Read more...

 
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