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Methods in cell-matrix adhesion

Author: Josephine C Adams; American Society for Cell Biology.
Publisher: Amsterdam : London : Academic, 2002.
Series: Methods in cell biology, v. 69.
Edition/Format:   Print book : EnglishView all editions and formats
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Methods in Cell-Matrix Adhesion contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This  Read more...

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Document Type: Book
All Authors / Contributors: Josephine C Adams; American Society for Cell Biology.
ISBN: 012044142X 9780120441426 0125441681 9780125441681
OCLC Number: 50176241
Notes: Prepared under the auspices of the American Society for Cell biology.
Description: xv, 461 pages, [9] pages of plates : illustrations ; 24 cm.
Contents: Preface and Perspectives on Cell-Matrix Adhesion --
Cell-Matrix Interactions: The View from the Outside / Paul Bornstein --
Matrix and Meaning / Martin A. Schwartz --
Cell-Matrix Adhesion Research and the Development of Biotherapeutics / Roy R. Lobb --
Matrix Methodologies --
Detection and Purification of Instructive Extracellular Matrix Components with Monoclonal Antibody Technologies / Susana G. Gil, Randy O. Sigle, William G. Carter --
Methods for Detecting ECM Components That Instruct Cell Responses in Reporter Cells --
Preparation of Mabs for Identification of Instructive ECM --
Purification of Instructive ECM with Mabs and Interactions with Reporter Cells --
Isolation and Purification of Proteoglycans / John M. Whitelock, Renato V. Iozzo --
Extraction of Proteoglycans --
Radiolabeling of Proteoglycans for Ease of Purification --
Anion-Exchange Chromatography --
Hydrophobic Interaction Chromatography --
Gel Filtration Chromatography --
Immunoaffinity Chromatography --
Quantification and Characterization --
Expression of Recombinant Matrix Components Using Baculoviruses / Deane F. Mosher, Kristin G. Huwiler, Tina M. Misenheimer, Douglas S. Annis --
Introduction: The Importance of Recombinant Extracellular Matrix Molecules for Protein Studies --
Requirements and Rationale for Development of a General Protein Expression System --
Expression in Insect Cells Using pCOCO --
Results Obtained from the COCO System --
Heparan Sulfate-Growth Factor Interactions / Alan C. Rapraeger --
Introduction: HS Regulation of Growth Factor Signaling --
Methods for Identifying the Role of HS in Growth Factor Binding and Signaling --
Identification of Regulatory HS --
Analyzing the Structure of HS General Considerations and Methodologies --
Analysis of Basement Membrane Self-Assembly and Cellular Interactions with Native and Recombinant Glycoproteins / Peter D. Yurchenco, Sergei Smirnov, Todd Mathus --
Native and Recombinant Basement Membrane Proteins --
Analysis of Self-Assembly in Vitro --
Analysis of Cell-Surface Contributions to Basement Membrane Assembly --
Approaches to Evaluating ECM Molecules for Assembly Interactions --
Integration of Genetic and Molecular Approaches --
Preparation and Analysis of Synthetic Multicomponent Extracellular Matrix / Kim S. Midwood, Iwona Wierzbicka-Patynowski, Jean E. Schwarzbauer --
Introduction to the ECM --
Development of Synthetic Matrices --
Analysis of Matrix Dynamics by Atomic Force Microscopy / Helen G. Hansma, Dennis O. Clegg, Efrosini Kokkoli, Emin Oroudjev, Matthew Tirrell --
General Experimental Design --
Probe Microscopy in Fluid: AFM Imaging --
Probe Microscopy in Fluid: Molecular Pulling/Force Spectroscopy --
Probe Microscopy in Air: AFM Imaging --
Analysis of Matrix Degradation / H. G. Munshi, M. Sharon Stack --
Experimental Approaches and Protocols --
Adhesion Receptor Methodologies --
Expression Cloning Strategies for the Identification of Adhesion Molecules / Joe W. Ramos, Mark Ginsberg --
Importance of Methodology --
Protocols --
Points of Variation --
Critical Controls --
Potential Use in Disease/Drug Screening Applications --
Purification of Integrins and Characterization of Integrin-Associated Proteins / Johannes A. Eble, Fedor Berditchevski --
Isolation of Integrins: General Considerations and Points of Variation --
Characterization of Integrin-Associated Proteins through mAb Production: General Considerations and Points of Variation --
Methods for Analysis of the Integrin Ligand Binding Event / Jeffrey W. Smith --
Application of Affinity Cross-Linking to Study of the Integrin Ligand Binding Pocket --
Microtiter-Based Integrin Binding Assays --
Measuring Ligand Binding to Integrins with Surface Plasmon Resonance --
Analyzing Integrin Redox Status --
Intracellular Coupling of Adhesion Receptors: Molecular Proximity Measurements / Maddy Parsons, Tony Ng --
Introduction and Applications --
Fluorescent Lifetime Imaging Microscopy (FLIM) --
Functional Applications of Cell-Matrix Adhesion in Molecular Cell Biology --
Functional Analysis of Cell Adhesion: Quantitation of Cell-Matrix Attachment / Steven K. Akiyama --
General Concepts in Cell Adhesion Assays --
Cell Attachment Assays --
Cell Spreading Assays --
Direct Binding Assays --
Cell Migration Assays --
Measurements of Glycosaminoglycan-Based Cell Interactions / J. Kevin Langford, Ralph D. Sanderson --
Cell Adhesion Assay --
Cell Invasion Assay --
Applications of Adhesion Molecule Gene Knockout Cell Lines / Jordan A. Kreidberg --
Derivation of Somatic Cell Lines --
ES Cell Lines Carrying Homozygous Mutations of Integrin Adhesion Molecule Genes --
Studies with Adhesion Receptor Null Cell Lines --
Studies with Extracellular Matrix Component Null Cell Lines --
Flexible Polyacrylamide Substrata for the Analysis of Mechanical Interactions at Cell-Substratum Adhesions / Karen A. Beningo, Chun-Min Lo, Yu-Li Wang --
Preparation of the Polyacrylamide Substratum --
Characterization of the Gel --
Data Collection for the Analysis of Traction Forces --
Analysis of Traction Forces --
Other Applications Using Polyacrylamide Substrata --
Cell Migration in Slice Cultures / Donna J. Webb, Hannelore Asmussen, Shin-ichi Murase, Alan F. Horwitz --
Somitic Migration --
Migration from the Subventricular Zone to the Olfactory Bulb --
Axonal Outgrowth --
Conclusions and Perspectives --
Application of Cell Adhesion to Study Signaling Networks / Cindy K. Miranti --
Methods --
Use of Micropatterned Adhesive Surfaces for Control of Cell Behavior / Philip LeDuc, Emanuele Ostuni, George Whitesides, Donald Ingber --
Materials and Instrumentation --
Procedures --
Pitfalls --
Adenoviral-Mediated Gene Transfer in Two-Dimensional and Three-Dimensional Cultures of Mammary Epithelial Cells / Harriet Watkin, Charles H. Streuli --
Using Collagen I as a Three-Dimensional Matrix for Cell Culture --
Using Reconstituted Basement Membrane as a Three-Dimensional Matrix for Cell Culture --
Preparing Mouse Mammary Epithelial Cell Cultures --
Preparing Recombinant Adenovirus for Infecting Mammary Epithelial Cells --
Infecting Mammary Epithelial Cell Cultures with Adenovirus.
Series Title: Methods in cell biology, v. 69.
Responsibility: edited by Josephine C. Adams.

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Iozzo -- Extraction of Proteoglycans -- Radiolabeling of Proteoglycans for Ease of Purification -- Anion-Exchange Chromatography -- Hydrophobic Interaction Chromatography -- Gel Filtration Chromatography -- Immunoaffinity Chromatography -- Quantification and Characterization -- Expression of Recombinant Matrix Components Using Baculoviruses / Deane F. Mosher, Kristin G. Huwiler, Tina M. Misenheimer, Douglas S. Annis -- Introduction: The Importance of Recombinant Extracellular Matrix Molecules for Protein Studies -- Requirements and Rationale for Development of a General Protein Expression System -- Expression in Insect Cells Using pCOCO -- Results Obtained from the COCO System -- Heparan Sulfate-Growth Factor Interactions / Alan C. Rapraeger -- Introduction: HS Regulation of Growth Factor Signaling -- Methods for Identifying the Role of HS in Growth Factor Binding and Signaling -- Identification of Regulatory HS -- Analyzing the Structure of HS General Considerations and Methodologies -- Analysis of Basement Membrane Self-Assembly and Cellular Interactions with Native and Recombinant Glycoproteins / Peter D. Yurchenco, Sergei Smirnov, Todd Mathus -- Native and Recombinant Basement Membrane Proteins -- Analysis of Self-Assembly in Vitro -- Analysis of Cell-Surface Contributions to Basement Membrane Assembly -- Approaches to Evaluating ECM Molecules for Assembly Interactions -- Integration of Genetic and Molecular Approaches -- Preparation and Analysis of Synthetic Multicomponent Extracellular Matrix / Kim S. Midwood, Iwona Wierzbicka-Patynowski, Jean E. Schwarzbauer -- Introduction to the ECM -- Development of Synthetic Matrices -- Analysis of Matrix Dynamics by Atomic Force Microscopy / Helen G. 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