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Methods in mouse atherosclerosis

Author: Beatriz Dorado; Vicente Andrés
Publisher: New York : Humana Press : Springer, ©2015.
Series: Methods in molecular biology (Clifton, N.J.), v. 1339; Springer protocols (Series)
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and  Read more...
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Genre/Form: Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Methods in mouse atherosclerosis.
New York : Humana Press, [2015]
(DLC) 2015948251
(OCoLC)908374588
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Beatriz Dorado; Vicente Andrés
ISBN: 9781493929290 1493929291 1493929283 9781493929283
OCLC Number: 927456076
Description: 1 online resource (xiv, 400 pages) : illustrations (some color).
Contents: Use of mouse models in atherosclerosis research / Godfrey S. Getz and Catherine A. Reardon --
Cytokines and immune responses in murine atherosclerosis / Pascal J.H. Kusters and Esther Lutgens --
Intravital microscopy for atherosclerosis research / Remco T.A. Megens and Oliver Soehnlein --
Noninvasive molecular imaging of mouse atherosclerosis / Martina A. McAteer and Robin P. Choudhury --
Oil red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root / M. Jesús Andrés-Manzano, Vicente Andrés, and Beatriz Dorado --
Isolation, culture, and polarization of murine bone marrow-derived and peritoneal macrophages / Inés Pineda-Torra [and others] --
Isolation of mouse primary aortic endothelial cells by selection with specific antibodies / Pedro Molina-Sánchez and Vicente Andrés --
Isolation and culture of aortic smooth muscle cells and in vitro calcification assay / Ricardo Villa-Bellosta and Magda R. Hamczyk --
Immunostaining of macrophages, endothelial cells, and smooth muscle cells in the atherosclerotic mouse aorta / Prashanthi Menon and Edward A. Fisher --
Immunostaining of lymphocytes in mouse atherosclerotic plaque / Anton Gisterå and Daniel F.J. Ketelhuth --
Flow cytometric analysis of immune cells within murine aorta / Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, and Elena V. Galkina --
In vitro differentiation of naïve CD4+ T cells : a tool for understanding the development of atherosclerosis / Salvador Iborra and Jose M. González-Granado --
Quantification of apoptosis in mouse atherosclerotic lesions / Nichola L. Figg and Martin R. Bennett --
Quantification of cellular proliferation in mouse atherosclerotic lesions / José J. Fuster --
Quantification of in vitro macrophage cholesterol efflux and in vivo macrophage-specific reverse cholesterol transport / Joan Carles Escolà-Gil [and others] --
In vitro macrophage phagocytosis assay / Magda R. Hamczyk, Ricardo Villa-Bellosta, and Vicente Andrés --
Glucose and insulin tolerance tests in the mouse / Ángela Vinué and Herminia González-Navarro --
Wire myography to study vascular tone and vascular structure of isolated mouse arteries / Lara del Campo and Mercedes Ferrer --
Pressure myography to study the function and structure of isolated small arteries / Olav L. Schjørring, Rune Carlsson, and Ulf Simonsen --
Generation of aorta transcript atlases of wild-type and apolipoprotein E-null mice by laser capture microdissection-rased mRNA expression microarrays / Changjun Yin [and others] --
Analysis of gene and protein expression in atherosclerotic mouse aorta by western blot and quantitative real-time PCR / José Rivera-Torres --
Bone marrow transplantation in mice to study the role of hematopoietic cells in atherosclerosis / Vinatha Sreeramkumar and Andrés Hidalgo --
Tandem stenosis to induce atherosclerotic plaque instability in the mouse / Yung Chih Chen, Jennifer Rivera, and Karlheinz Peter --
Detection of intraplaque hemorrhage in mouse atherosclerotic lesions / Judith C. Sluimer, Marion J. Gijbels, and Sylvia Heeneman --
Mechanical stabilization of mouse carotid artery for in vivo intravital microscopy imaging of atherogenesis / Raphaël Chèvre --
Intravital microscopy in the cremaster muscle microcirculation for endothelial dysfunction studies / Cristina Rius and María J. Sanz --
Fluorescent molecular tomography for in vivo imaging of mouse atherosclerosis / Alicia Arranz [and others] --
In vivo 18F-FDG-PET imaging in mouse atherosclerosis / Jesús Mateo [and others] --
Magnetic resonance imaging of the atherosclerotic mouse aorta / Jesús Mateo [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1339; Springer protocols (Series)
Responsibility: edited by Vicente Andrés, Beatriz Dorado.

Abstract:

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive  Read more...

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