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Methods in neurosciences. Volume 15, Photoreceptor cells

Publisher: San Diego, California : Academic Press, [1993] ©1993
Series: Methods in neurosciences, v. 15.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

Advances in the study of photoreceptor cells, which have proceeded at a rapid pace, have increased our understanding of cell functions. This volume presents up-to-date biochemical, cell and  Read more...

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Genre/Form: Electronic books
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
ISBN: 9781483217543 148321754X 1322054371 9781322054377
OCLC Number: 891395736
Description: 1 online resource (425 pages) : illustrations.
Contents: Cover image; Title page; Table of Contents; Methods in Neurosciences; Copyright page; Contributors to Volume 15; Preface; Methods in Neurosciences; Section I: Structure of Photoreceptor Cells; Chapter 1: Light and Electron Microscopy of Vertebrate Photoreceptors; Publisher Summary; Introduction; Overview of Photoreceptor Cell Structure; Methods; Acknowledgments; Chapter 2: Rapid Freezing and Subsequent Preparation Methods in Retinal Cell Biology; Publisher Summary; Introduction; Rapid Freezing; Freeze-substitution; Freeze Replicas; Freeze-Drying. Chapter 3: Preparation of Retinas for Studying Photoreceptors with Confocal MicroscopyPublisher Summary; Introduction; Overview of Methodology; Reagents; Procedures; Acknowledgment; Section II: Metabolic Studies; Chapter 4: In Vivo Labeling of Retinal Components by Intraocular Injection; Publisher Summary; Introduction; Methods; Selected Results; Chapter 5: In Vitro Biosynthetic Studies with Isolated Vertebrate Retinas; Publisher Summary; Introduction; Methods; Selected Results; Acknowledgments; Chapter 6: Isolation of Post-Golgi Membranes Transporting Newly Synthesized Rhodopsin. Publisher SummaryIntroduction; Subcellular Fractionation of Retinal Tissue; Conclusion; Section III: Photoreceptor Membrane and Protein Preparation and Characterization; Chapter 7: Preparing Rod Outer Segment Membranes, Regenerating Rhodopsin, and Determining Rhodopsin Concentration; Publisher Summary; Introduction; Rod Outer Segment Preparation; Preparation of Rod Outer Segments from Other Species; Measuring the Concentration of Rhodopsin; Regeneration of Rhodopsin; Summary; Acknowledgments; Chapter 8: Isolation and Characterization of Rod Outer Segment Disk and Plasma Membranes. Publisher SummaryIntroduction; Purification of Intact Rod Outer Segments; Separation of Rod Outer Segments into Soluble and Membrane Fractions; Separation of Rod Outer Segment Disk and Plasma Membranes; Characterization of Rod Outer Segment Disk and Plasma Membranes; Conclusions; Acknowledgments; Chapter 9: Immunological Measurement of Rhodopsin; Publisher Summary; Introduction; Assay Methods for Rhodopsin; Chapter 10: Preparation and Characterization of Chicken Rod and Cone Pigments; Publisher Summary; Introduction; Overview of Procedures; Experimental Procedures; Discussion; Acknowledgments. Chapter 11: Purification and Characterization of Bovine Transducin and its SubunitsPublisher Summary; Introduction; Purification of Transducin and Its Subunits; Functional Assays for Transducin; Chapter 12: Purification and Characterization of Phosducin from Bovine Retina; Publisher Summary; Introduction; Assay Method; Purification Procedure; Properties of Bovine Transducin; Acknowledgments; Chapter 13: pH Assay of Rod Outer Segment Cyclic GMP Phosphodiesterase Activity; Publisher Summary; Background; Methods; Factors Affecting Phosphodiesterase Activation and Inactivation.
Series Title: Methods in neurosciences, v. 15.
Responsibility: edited by Paul A. Hargrave, Department of Ophthalmology, University of Florida, Gainesville, Florida.

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