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Methods of Cell Separation

Author: Nicholas Catsimpoolas
Publisher: Boston, MA : Springer US, 1977.
Series: Biological Separations.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Nicholas Catsimpoolas
ISBN: 9781468408201 1468408208 9781468408225 1468408224
OCLC Number: 840287532
Description: 1 online resource.
Contents: 1 Preparative Density Gradient Electrophoresis and Velocity Sedimentation at Unit Gravity of Mammalian Cells --
I. Density Gradient Electrophoresis --
II. Velocity Sedimentation at Unit Gravity --
III. Conclusions --
References --
2 Isopycnic Separation of Cells and Cell Organelles by Centrifugation in Modified Colloidal Silica Gradients --
I. Introduction --
II. Preparations of Colloidal Silica --
III. Methodology --
IV. Centrifugation of Cells --
V. Centrifugation of Subcellular Particles --
VI. Concluding Remarks --
References --
3 Dielectrophoresis: Applications to the Characterization and Separation of Cells --
I. Introduction --
II. Mechanism of Nonuniform Field Effects and Dielectrophoresis --
III. Polarization Mechanisms in Biological Materials --
IV. Experimental Collection and Separation of Cells --
References --
4 Separation of Viable Cells by Velocity Sedimentation in an Isokinetic Gradient of Ficoll in Tissue Culture Medium --
I. Introduction --
II. Historical Development of Technique --
III. Isopycnic Sedimentation --
IV. Velocity (Including Isokinetic) Sedimentation in Ficoll Gradients --
V. Selected Theoretical Considerations --
VI. Critical Analysis of Data from Experiments in Cell Separation --
References --
5 Fractionation and Manipulation of Cells with Chemically Modified Fibers and Surfaces --
I. Introduction --
II. Affinity Fractionation of Cells --
III. Fiber Fractionation of Cells --
IV. Manipulation of Cells and the Study of Localized Perturbations at the Cell Surface --
V. Procedures --
VI. Applications --
VII. Prospective Applications --
References --
6 The Separation of Lymphoid Cells on the Basis of Physical Parameters: Separation of B- and T-Cell Subsets and Characterization of B-Cell Differentiation Stages --
I. Introduction --
II. Separation of B from T Lymphocytes --
III. Separation of Functionally Distinct B-Lymphocyte Subsets --
IV. General Conclusions --
V. Appendix: Technical Aspects of the Separation Procedures --
References --
7 Multiparameter Analysis and Sorting of Mammalian Cells --
I. Introduction --
II. Description of the LASL Multiparameter Cell Separator --
III. Examples of Multiparameter Cell Analysis and Sorting Applications --
IV. Summary and Conclusions --
References --
8 Partition of Cells in Two-Polymer Aqueous Phases: A Surface Affinity Method for Cell Separation --
I. Introduction --
II. Methods --
III. Results --
IV. Conclusion --
References.
Series Title: Biological Separations.
Responsibility: edited by Nicholas Catsimpoolas.

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