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Microbes as tools for cell biology

Author: David G Russell; American Society for Cell Biology.
Publisher: San Diego : Academic Press, ©1994.
Series: Methods in cell biology, v. 45.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Microbes as tools for cell biology.
San Diego : Academic Press, ©1994
(DLC) 95117439
(OCoLC)32275830
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: David G Russell; American Society for Cell Biology.
ISBN: 9780080859439 0080859437 012564146X 9780125641463 1281755621 9781281755629
OCLC Number: 298324951
Notes: "Prepared under the auspices of the American Society for Cell Biology."
Description: 1 online resource (xvi, 339 pages) : illustrations.
Contents: Front Cover; Microbes as Tools for Cell Biology; Copyright Page; Contents; Contributors; Preface; Introduction; Chapter 1. Obtaining and Maintaining Microbial Pathogens; I. Introduction; II. Biosafety Considerations; III. Department of Health and Human Services Guidelines; IV. Federal and Institutional Requirements; V. Source of Pathogens; VI. Culture and Maintenance of Pathogens; VII. Conclusion; Part I: Manipulation of Pathogens; Chapter 2. Cultivation of Malaria Parasites; I. Introduction; II. Culture Medium; III. Serum; IV. Erythrocytes; V. Culture Systems; VI. Synchronization. VII. Gametocyte Production in CultureVIII. Cloning Methods; IX. Cryopreservation; X. Serum Replacement; XI. Axenic (Extracellular) Development of Erythrocytic Stages of P. falciparum; XII. Cultivation of Erythrocytic Stages of Other Species of Malaria Parasites; XIII. In Vitro Development of Preerythrocytic and Sporogonic Stages of the Life Cycle; References; Chapter 3. Molecular Tools for Genetic Dissection of the Protozoan Parasite Toxoplasma gondii; I. Introductory Overview; II. In Vitro Culture of T. gondii Tachyzoites; III. Molecular Transformation Systems for Toxoplasma. IV. Summary and OutlookReferences; Chapter 4. Transfection Experiments with Leishmania; I. Introduction; II. Stable and Transient Transfection; III. Transfection; References; Chapter 5. Mutagenesis and Variant Selection in Salmonella; I. Introduction; II. Mutagenesis; III. Screening of Variants; IV. Genetic Analysis; V. Perspectives; References; Chapter 6. Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection; I. Introduction; II. Biosafety Considerations; III. Culture Media and Conditions; IV. Isolation of Mycobacteria; V. Maintenance of Stocks; VI. Genetic Techniques. VII. MutagenesisVIII. Strain Construction; IX. Mutant Selection and Isolation; References; Part II: Microbial Adherence and Invasion Assays; Chapter 7. Modulation of Murine Macrophage Behavior in Vivo and in Vitro; I. Introduction; II. Reagents and Solutions; III. Animal Husbandry and Maintenance of Immunocompromised Mice; IV. Eliciting and Harvesting Macrophages in Vivo; V. Adherence of Macrophages to Solid Substrates; VI. Assays for Macrophage Phagocytic Activity; VII. Analysis of MHC Class II Antigen Expression; References. Chapter 8. In Vitro Assays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal TransductionI. Introduction; II. Phagocytosis Assays; III. Phagocytic Receptors; IV. Stimulation of Phagocytosis; V. Phagosome Isolation; References; Chapter 9. Bacterial Adhesion and Colonization Assays; I. Introduction; II. In Situ Screening of Host Receptor Distribution; III. Bacterial Adherence to Cells in Culture; IV. Biochemical Characterization of the Molecular Nature of Receptors in Situ; V. Bacterial Inhibition Experiments in Situ.
Series Title: Methods in cell biology, v. 45.
Responsibility: edited by David G. Russell.
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Abstract:

Provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. This volume is organized into three sections, covering essential information on culture  Read more...

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"Microbes as Tools for Cell Biology contains a potpourri of techniques."--TRENDS IN MICROBIOLOGY

 
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