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Microfluidic cell culture systems

Author: Christopher Bettinger; Jeffrey T Borenstein; Sarah L Tao
Publisher: Amsterdam : Elsevier, William Andrew, 2013.
Series: Micro & nano technologies.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
The fields of microfluidics and BioMEMS are signigicantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. One of the key benefits  Read more...
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Genre/Form: Electronic books
Handbooks, manuals, etc
Additional Physical Format: Print version:
Bettinger, Christopher.
Microfluidic Cell Culture Systems.
Burlington : Elsevier Science, ©2012
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Christopher Bettinger; Jeffrey T Borenstein; Sarah L Tao
ISBN: 9781437734607 143773460X 1437734596 9781437734591
OCLC Number: 826898602
Notes: William Andrew is an imprint of Elsevier.
Description: 1 online resource (PDF, XVIII, 433 pages) : illustrations.
Contents: Front Cover; Microfluidic Cell Culture Systems; Copyright page; Contents; Preface; List of Contributors; 1 Materials and Fabrication Methods; 1 Microfluidic Cell Culture Platforms with Embedded Nanoscale Features; 1.1 Introduction; 1.2 Engineering of nanoscale features; 1.2.1 Fabrication of irregular nanoscale features; 1.2.2 Fabrication of regular nanoscale features; 1.2.2.1 Focused beam lithography; 1.2.2.2 Replication techniques; 1.2.2.3 Stitching technique; 1.3 Assembly of PDMS-based microfluidic platforms; 1.3.1 Reversible assembly; 1.3.2 Irreversible assembly. 1.3.3 Microtransfer assembly1.4 Microfluidic platforms with embedded nanoscale features for cell studies; 1.4.1 Cancer cell detection; 1.4.2 Stem cell regulation; 1.5 Summary; Acknowledgment; References; 2 Microvascular Networks for Tissue Engineering; 2.1 Introduction; 2.2 Characteristics of branched vascular networks; 2.3 Fabrication of 2-D microvascular networks; 2.4 Fabrication of 3-D microvascular networks; 2.5 Microchannel topologies; 2.6 Engineering meets biology: toward tissue engineering applications; 2.7 Outlook and future challenges; Acknowledgments; References. 3 Microfluidics for Engineering 3D Tissues and Cellular Microenvironments3.1 Introduction; 3.2 Fabricating 3D tissue scaffolds using microfluidics; 3.2.1 Microfluidic casting for engineering the microstructure of natural ECM scaffolds; 3.2.2 Fabrication of composite photopolymerizable hydrogel microstructures using microfluidics; 3.3 Dynamic 3D cell cultures within PDMS microfluidic devices; 3.4 Hydrogel-based microfluidic culture devices and tissue scaffolds; 3.4.1 Assembly of natural ECM multiphase tissues using microfluidics. 3.4.2 Optimization of collagen-fiber interfacing for directing capillary morphogenesis within microfabricated tissue constructs3.5 Conclusion and future directions; References; 4 Fabrication of Advanced Microcontainer Arrays for Perfused 3D Cell Culture in Microfluidic Bioreactors; 4.1 Introduction; 4.2 Micromolding of cell container arrays; 4.2.1 Micro injection molding; 4.2.2 Hot embossing; 4.2.3 Microscale thermoforming; 4.2.3.1 Micro pressure forming; 4.2.3.2 Other microthermoforming processes; 4.3 Introducing porosity; 4.3.1 Laser micromachining. 4.3.2 Solvent vapor bonding of track-etched membranes4.3.3 Combination of thermoforming and ion track technology; 4.4 Functionalization of cell container arrays; 4.4.1 Protein coatings; 4.4.2 SMART processes for overlaid patterned surface and bulk modification of thermoformed cell container arrays; 4.4.2.1 Cell adhesion micropatterns; 4.4.2.2 Surface and bulk micro- and nano topographies; 4.4.2.3 Microelectrodes; 4.5 Integration into microfluidic bioreactors; 4.6 Conclusion; References; 5 Mechanobiological Approaches for the Control of Cell Motility; 5.1 Introduction.
Series Title: Micro & nano technologies.
Responsibility: editers Christopher Bettinger, Jeffrey T. Borenstein, Sarah L. Tao.

Abstract:

A guide to the principles of microfluidic device design and operation, and their application to cell culture techniques. It provides those involved in traditional microfluidics and bioMEMS with an  Read more...

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