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MicroRNA expression detection methods

Author: Zhiguo Wang, Dr.; Baofeng Yang; SpringerLink (Online service)
Publisher: Berlin ; London : Springer, 2010.
Series: Springer eBook collection.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression, their application in miRNA research and their potential as tools for clinical diagnosis and prognosis. It begins with the introduction of the overall concept and strategies of miRNA expression detection methods emphasizing the need of a wide variety of these methods to suit specific  Read more...
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Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Zhiguo Wang, Dr.; Baofeng Yang; SpringerLink (Online service)
ISBN: 9783642049286 3642049281
OCLC Number: 604703933
Reproduction Notes: Electronic reproduction. New York : Springer, 2010. Mode of access: World Wide Web. Title from title screen (viewed on Mar. 30, 2010). Made available through SpringerLink.
Description: xx, 387 pages : illustrations ; 24 cm
Contents: General Remarks --
Detection, Profiling, and Quantification of miRNA Expression --
miRNA Microarray Methods --
Microarray and Its Variants for miRNA Profiling --
Northern Blotting Methods --
Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs --
In Situ Hybridization Methods --
In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs --
Real-Time RT-PCR Methods --
End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification --
miR-Q RT-PCR for miRNA Quantification --
Poly(A)-Tailed Universal Reverse Transcription --
Multiplexing RT-PCR for High-Throughput miRNA Profiling --
miRNA Amplification Profiling (mRAP) --
Cloning Methods --
miRNA Serial Analysis of Gene Expression (miRAGE or SAGE) --
Nanoparticle Methods --
Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis --
Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling --
Conducting Polymer Nanowires Technique for High-Sensitivity miRNA Expression Analysis --
Gold Nanoparticle Probe Method for miRNA Quantification --
Other Methods --
Splinted Ligation Method --
Padlock-Probes and Rolling-Circle Amplification --
Invader Assay --
Single Molecule Method --
Enzymatic Method --
Surface-Enhanced Raman Spectroscopy Method --
RAKE Assay --
Bead-Based Flow Cytometric miRNA Expression Profiling --
Bioluminescence miRNA Detection Method --
Molecular Beacon Method --
Ribozyme Method --
Electrocatalytic Moiety Labeling Technique for High-Sensitivity miRNA Expression Analysis --
Circulating miRNA Detection Methods --
Serum and Plasma miRNA Detection --
miRNA Detection from Peripheral Blood Microvesicles --
Detection of Placental miRNAs in Maternal Plasma --
Single-cell miRNA Detection Methods --
Quantitative LNA-ELF-FISH Method for miRNA Detection in Single Mammalian Cell --
Single Cell Stem-Looped Real-Time PCR --
miRNA Function-Reporter Expression Assay --
Whole Mount In Situ Analysis --
Whole Mount In Situ Hybridization (WM-ISH) for miRNA Expression Profiling During Vertebrate Development.
Series Title: Springer eBook collection.
Responsibility: Zhiguo Wang, Baofeng Yang.

Abstract:

This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression as well as their application in miRNA research and their potential as tools  Read more...

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From the reviews:"Designed primarily as a reference for life-science researchers, this book enables investigators to make unbiased comparisons in the search for the best technique for their unique Read more...

 
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