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MicroRNAs in development : methods and protocols

Author: Tamas Dalmay
Publisher: [New York] : Humana Press, ©2011.
Series: Methods in molecular biology (Clifton, N.J.), v. 732.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression,  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
MicroRNAs in development.
New York : Springer, ©2011
(OCoLC)711076365
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Tamas Dalmay
ISBN: 9781617790836 1617790834
OCLC Number: 719367741
Description: 1 online resource (x, 227 pages).
Contents: In situ detection of microRNAs in animals / Dylan Sweetman --
Detection of microRNAs in plants by in situ hybridisation / Eva Varallyay and Zoltan Havelda --
Detecting sRNAs by northern blotting / Sara Lopez-Gomollon --
Profiling microRNAs by real-time PCR / Nanna Jacobsen, Ditte Andreasen, and Peter Mouritzen --
Detection of small RNAs and microRNAs using deep sequencing technology / Ericka R. Havecker --
Detection of microRNAs in prostate cancer cells by mircoRNA array / Xiaoqing Tang [and others] --
MicroRNA knock down by cholesterol-conjugated antisense oligos in mouse organ culture / Sharon Kredo-Russo and Eran Hornstein --
Protocols for use of homologous recombination gene targeting to produce microRNA mutants in drosophila / Ya-Wen Chen, Ruifen Weng, and Stephen M. Cohen --
Engineering elements for gene silencing : the artificial microRNAs technology / Pablo Andres Manavella and Ignacio Rubio-Somoza --
Mimicry technology : suppressing small RNA activity in plants / Ignacio Rubio-Somoza and Pablo Andres Manavella --
Experimental validation of microRNA targets using a luciferase reporter system / Francisco E. Nicolas --
Experimental identification of microRNA targets by immunoprecipitation of argonaute protein complexes / Michaela Beitzinger and Gunter Meister --
Comprehensive identification of miRNA target sites in live animals / Dimitrios G. Zisoulis, Gene W. Yeo, and Amy E. Pasquinelli --
Target validation of plant microRNAs / Cesar Llave [and others] --
High-throughput sequencing-based methodology to identify all uncapped and cleaved RNA molecules in eukaryotic genomes / Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 732.
Responsibility: edited by Tamas Dalmay.

Abstract:

MicroRNAs in Development explores how to detect and profile miRNA expression, and outlines how to identify and validate miRNA targets in animals and plants.  Read more...

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From the reviews: "Well edited book by Prof. Tamas Dalmay provides a complete (as for today !) overview of the role played by microRNAs ... . Thanks to this book I think that a multitude of readers Read more...

 
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