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Microscopy Applied to Biophotonics.

Author: F S Pavone; P T C So; P M W French
Publisher: Burke : IOS Press, 2014.
Series: '">International School of Physics "Enrico Fermi."
Edition/Format:   eBook : Document : English
Summary:
Biophotonics and microscopy are highly inter-related fields in terms of both technological development and biomedical applications. Recent advances in microscopy have been paralleled by new opportunities for biophotonics, including the investigation and manipulation of biological phenomena using light and its application to biomedicine. This book contains papers from the Enrico Fermi International School of Physics  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Pavone, F.S.
Microscopy Applied to Biophotonics.
Burke : IOS Press, ©2014
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: F S Pavone; P T C So; P M W French
ISBN: 9781614994138 1614994137
OCLC Number: 923709696
Notes: ""Fundamentals PT and PA techniques""
Description: 1 online resource (219 pages).
Contents: ""Title Page""; ""Contents""; ""Preface""; ""Course group shot""; ""Overview of fluorescence imaging for biophotonics""; ""Optical microscopes""; ""Improved image quality and 3D imaging""; ""Functional imaging using fluorescence""; ""Beyond the diffraction limit""; ""High-speed and throughput""; ""Imaging in biological tissue""; ""Conclusions""; ""Nonlinear imaging of tissues""; ""Introduction""; ""Theoretical background""; ""Two-photon excitation fluorescence microscopy""; ""Second-harmonic generation microscopy""; ""Fluorescence lifetime imaging microscopy""; ""Morphological imaging"" ""Combined two-photon fluorescence?second-harmonic generation microscopy on skin tissue""""Combined two-photon fluorescence?second-harmonic generation microscopy on diseased dermis tissue""; ""Combined two-photon fluorescence?second-harmonic generation microscopy on bladder tissue""; ""Second-harmonic generation imaging on cornea""; ""Improving the penetration depth with two-photon imaging: application of Optical Clearing Agents""; ""Chemical imaging""; ""Lifetime imaging of Basal Cell Carcinoma""; ""Enhancing tumor margins with two-photon fluorescence by using aminolevulinic acid"" ""Morpho-functional imaging""""Single spine imaging and ablation inside brain of small living animals""; ""Optical recording of electrical activity in intact neuronal network by random access second-harmonic (RASH) microscopy""; ""Conclusion""; ""New microscopy for the 3D visualization of large samples with highest resolution""; ""Status of research of ultramicroscopy""; ""3D visualization of fixed specimens""; ""Visualization of neurons""; ""Visualization of mouse embryos""; ""Visualization of flies""; ""Study of the development of the vascular system in the mouse embryo"" ""High dynamic range imaging""""Raman spectroscopy: A powerful tool for biomedical applications""; ""Introduction and theoretical background""; ""Results and discussions""; ""Biomedical SERS approaches""; ""Rapid identification of microorganisms""; ""Cell and tissue diagnostics via linear and non-linear Raman imaging""; ""Conclusion""; ""In vivo optical flow cytometry and cell imaging""; ""Goal and motivation""; ""Introduction""; ""In vitro conventional flow cytometry""; ""Intravital video microscopy""; ""Animal models""; ""In vivo fluorescence flow cytometry (IVFFC)"" ""Single-photon intravital fluorescence flow cytometry (SPIVFFC)""""Multiphoton intravital fluorescence flow cytometry (MPIVFFC)""; ""Label-free back-reflectance in vivo cytometry""; ""In vivo cytometry based on transmittance digital microscopy (TDM)""; ""Basic principles""; ""High-speed, high-resolution in vivo imaging cytometry""; ""Instrumentation and some applications""; ""Optical clearing for in vivo label-free image cytometry""; ""Cell velocity measurements using TDM""; ""Summary""; ""Photothermal (PT) and photoacoustic (PA) flow cytometry techniques""
Series Title: International School of Physics "Enrico Fermi."

Abstract:

Biophotonics and microscopy are highly inter-related fields in terms of both technological development and biomedical applications. Recent advances in microscopy have been paralleled by new opportunities for biophotonics, including the investigation and manipulation of biological phenomena using light and its application to biomedicine. This book contains papers from the Enrico Fermi International School of Physics on Microscopy Applied to Biophotonics, held in Varenna, Italy, in July 2011. The lectures spanned the basic science of imaging, through advanced microscopy techniques, to the state-

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