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Mitochondria : practical protocols

Author: Dejana Mokranjac; Fabiana Perocchi
Publisher: New York : Humana Press ; Springer, [2017] ©2017
Series: Methods in molecular biology (Clifton, N.J.), v. 1567.
Edition/Format:   eBook : Document : English : Second editionView all editions and formats
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Mitochondria.
New York : Humana Press, c2017
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Dejana Mokranjac; Fabiana Perocchi
ISBN: 9781493968244 1493968246
OCLC Number: 975492468
Description: 1 online resource (xi, 409 pages) : illustrations.
Contents: Guide to computational methods for predicting mitochondrial localization / Su Sun and Bianca H. Habermann --
Isolation of functional mitochondria from cultured cells and mouse tissues / Jennifer Wettmarshausen and Fabiana Perocchi --
Isolation of mitochondria from Saccharomyces cerevisiae / Toshiaki Izawa and Ann-Katrin Unger --
Isolation of contact sites between inner and outer mitochondrial membranes / Max Harner --
Isolation of mitochondria-associated membranes (MAM) from mouse brain tissue / Bernadette Schreiner and Maria Ankarcrona --
Label-free quantitative analysis of mitochondrial proteomes using the multienzyme digestion-filter aided sample preparation (MED-FASP) and "Total Protein Approach" / Jacek R. Wiśniewski --
Quantitative analysis of glycerophospholipids in mitochondria by mass spectrometry / Takashi Tatsuta --
Detection of cysteine redox states in mitochondrial proteins in intact mammalian cells / Markus Habich and Jan Riemer --
Chemical crosslinking in intact mitochondria / Rupa Banerjee, Umut Günsel, and Dejana Mokranjac --
Reconstitution of mitochondrial membrane proteins into nanodiscs by cell-free expression / Ketan Malhotra and Nathan N. Alder --
Detection of dual targeting and dual function of mitochondrial proteins in yeast / Reut Ben-Menachem and Ophry Pines --
Localizing mRNAs encoding mitochondrial proteins in yeast by fluorescence microscopy and subcellular fractionation / Dmitry Zabezhinsky, Hannah Sperber, and Jeffrey E. Gerst --
Assessing mitochondrial bioenergetics in isolated mitochondria from various mouse tissues using seahorse XF96 analyzer / Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, and Holger Prokisch --
Application of FRET-based biosensor "ATeam" for visualization of ATP levels in the mitochondrial matrix of living mammalian cells / Tomoki Yoshida, Soaad Alfaqaan, Norio Sasaoka, and Hiromi Imamura --
Microplate-based bioluminescence assay of mitochondrial calcium uptake / María Teresa Alonso, Paloma Navas-Navarro, and Javier García-Sancho --
New imaging tools to analyze mitochondrial morphology in Caenorhabditis elegans / Saroj G. Regmi and Stéphane G. Rolland --
Single molecule tracking and localization of mitochondrial protein complexes in live cells / Timo Appelhans and Karin Busch --
Analysis of yeast mitochondria by electron microscopy / Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, and Benedikt Westermann --
Analysis of mitochondrial membrane protein complexes by electron cryo-tomography / Vicki A.M. Gold, Tobias Brandt, Laetitia Cavellini, Mickael M. Cohen, Raffaele Ieva, and Martin van der Laan --
Assays for Mitophagy in Yeast / Akinori Eiyama and Koji Okamoto --
Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans / Konstantinos Palikaras and Nektarios Tavernarakis --
Assessing mitochondrial unfolded protein response in mammalian cells / Fiona Durand and Nicholas Hoogenraad --
Analysis of mitochondrial RNA-processing defects in patient-derived tissues by qRT-PCR and RNAseq / Robert Kopajtich, Johannes A. Mayr, and Holger Prokisch --
Identification of disease-causing mutations by functional complementation of patient-derived fibroblast cell lines / Laura S. Kremer and Holger Prokisch.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1567.
Responsibility: edited by Dejana Mokranjac, Fabiana Perocchi.

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