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Mitochondria : practical protocols

Author: Dejana Mokranjac; Fabiana Perocchi
Publisher: New York : Humana Press imprint is published by Springer Nature, [2017] ©2017
Series: Methods in molecular biology (Clifton, N.J.), v. 1567.; Springer protocols (Series)
Edition/Format:   eBook : Document : English : Second editionView all editions and formats
Summary:
"This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary  Read more...
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Genre/Form: Laboratory manuals
Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Mitochondria.
New York, NY : Humana Press, [2017]
(DLC) 2017930957
(OCoLC)962005379
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Dejana Mokranjac; Fabiana Perocchi
ISBN: 9781493968244 1493968246
OCLC Number: 975492468
Description: 1 online resource (xi, 409 pages) : illustrations (some color).
Contents: Guide to computational methods for predicting mitochondrial localization / Su Sun and Bianca H. Habermann --
Isolation of functional mitochondria from cultured cells and mouse tissues / Jennifer Wettmarshausen and Fabiana Perocchi --
Isolation of mitochondria from Saccharomyces cerevisiae / Toshiaki Izawa and Ann-Katrin Unger --
Isolation of contact sites between inner and outer mitochondrial membranes / Max Harner --
Isolation of mitochondria-associated membranes (MAM) from mouse brain tissue / Bernadette Schreiner and Maria Ankarcrona --
Label-free quantitative analysis of mitochondrial proteomes using the multienzyme digestion-filter aided sample preparation (MED-FASP) and "Total Protein Approach" / Jacek R. Wiśniewski --
Quantitative analysis of glycerophospholipids in mitochondria by mass spectrometry / Takashi Tatsuta --
Detection of cysteine redox states in mitochondrial proteins in intact mammalian cells / Markus Habich and Jan Riemer --
Chemical crosslinking in intact mitochondria / Rupa Banerjee, Umut Günsel, and Dejana Mokranjac --
Reconstitution of mitochondrial membrane proteins into nanodiscs by cell-free expression / Ketan Malhotra and Nathan N. Alder --
Detection of dual targeting and dual function of mitochondrial proteins in yeast / Reut Ben-Menachem and Ophry Pines --
Localizing mRNAs encoding mitochondrial proteins in yeast by fluorescence microscopy and subcellular fractionation / Dmitry Zabezhinsky, Hannah Sperber, and Jeffrey E. Gerst --
Assessing mitochondrial bioenergetics in isolated mitochondria from various mouse tissues using seahorse XF96 analyzer / Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, and Holger Prokisch --
Application of FRET-based biosensor "ATeam" for visualization of ATP levels in the mitochondrial matrix of living mammalian cells / Tomoki Yoshida, Soaad Alfaqaan, Norio Sasaoka, and Hiromi Imamura --
Microplate-based bioluminescence assay of mitochondrial calcium uptake / María Teresa Alonso, Paloma Navas-Navarro, and Javier García-Sancho --
New imaging tools to analyze mitochondrial morphology in Caenorhabditis elegans / Saroj G. Regmi and Stéphane G. Rolland --
Single molecule tracking and localization of mitochondrial protein complexes in live cells / Timo Appelhans and Karin Busch --
Analysis of yeast mitochondria by electron microscopy / Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, and Benedikt Westermann --
Analysis of mitochondrial membrane protein complexes by electron cryo-tomography / Vicki A.M. Gold, Tobias Brandt, Laetitia Cavellini, Mickael M. Cohen, Raffaele Ieva, and Martin van der Laan --
Assays for Mitophagy in Yeast / Akinori Eiyama and Koji Okamoto --
Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans / Konstantinos Palikaras and Nektarios Tavernarakis --
Assessing mitochondrial unfolded protein response in mammalian cells / Fiona Durand and Nicholas Hoogenraad --
Analysis of mitochondrial RNA-processing defects in patient-derived tissues by qRT-PCR and RNAseq / Robert Kopajtich, Johannes A. Mayr, and Holger Prokisch --
Identification of disease-causing mutations by functional complementation of patient-derived fibroblast cell lines / Laura S. Kremer and Holger Prokisch.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1567.; Springer protocols (Series)
Other Titles: Mitochondria (New York, N.Y.)
Responsibility: edited by Dejana Mokranjac, Fabiana Perocchi.

Abstract:

"This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondria: Practical Protocols, Second Edition aims to be useful for beginners as well as for experienced researchers in the field"--Publisher's description.

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