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|Genre/Form:||Handbooks, manuals, etc
|All Authors / Contributors:||
Michael R Green; Joseph Sambrook
|ISBN:||9781936113415 1936113414 9781936113422 1936113422|
|Notes:||Rev. ed. of: Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell. 2001.|
|Description:||3 v. (xxxiii, 1890, 46 p.) : ill. (some col.) ; 29 cm.|
Part 1 : Essentials. Isolation and quantification of DNA ; Analysis of DNA ; Cloning and transformation with plasmid vectors ; Gateway recombinational cloning ; Working with bacterial artificial chromosomes and other high-capacity vectors ; Extraction, purification, and analysis of RNA from eukaryotic cells ; Polymerase chain reaction ; Bioinformatics --
Part 2 : Analysis and manipulation of DNA and RNA. Quantification of DNA and RNA by real-time polymerase chain reactions ; Nucleic acid platform technologies ; DNA sequencing ; Analysis of labeled DNA, RNA, and oligonucleotide probes ; Methods for vitro mutagenesis --
Part 3 : Introducing genes into cells. Introducing genes into cultured mammalian cells ; Introducing genes into mammalian cells : viral vectors --
Part 4 : Gene expression. Analysis of gene regulation using reporter systems ; RNA interference and small RNA analysis ; Expressing cloned genes for protein production, purification, and analysis --
Part 5 : Interaction analysis. Cross-linking technologies for analysis of chromatin structure and function ; Mapping of in vivo RNA-binding sites by UV-cross-linking immunoprecipitation (CLIP) --
Appendices. Reagents and buffers ; Commonly used techniques ; Detection systems ; General safety and hazardous material.
|Responsibility:||Michael R. Green, Joseph Sambrook.|
Praise for the previous edition: "Any basic research laboratory using molecular biology techniques will benefit from having a copy on hand of the newly published Third Edition of Molecular Cloning: A