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Molecular genetics of asthma

Author: Maria Isidoro-Garcia
Publisher: New York, NY : Humana Press ; Springer, 2016.
Series: Methods in molecular biology (Clifton, N.J.), 1434.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their  Read more...
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Details

Genre/Form: Electronic books
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Maria Isidoro-Garcia
ISBN: 9781493936526 1493936522
OCLC Number: 951781110
Description: 1 online resource (xv, 275 pages) : illustrations (some color).
Contents: Applications of molecular genetics to the study of asthma / Catalina S. Sanz - Lozano --
Assessment and validation of new genetic variants : a systematic in silico approach/ Belén García-Berrocal, Asunción García-Sánchez, and María Isidoro-García --
Introduction to the gene expression analysis / Ignacio San Segundo-Val and Catalina S. Sanz-Lozano --
Real-time PCR for gene expression quantification in asthma / Ignacio San Segundo-Val, Virginia García-Solaesa, and Asunción García-Sánchez --
Validation of reference genes in mRNA expression analysis applied to the study of asthma / Ignacio San Segundo-Val and Catalina S. Sanz-Lozano --
Review of methods to study gene expression regulation applied to asthma / Asunción García-Sánchez and Fernando Marqués-García --
Interactions of DNA and proteins : electrophoretic mobility shift assay in asthma / Virginia García-Solaesa and Catalina S. Sanz-Lozano --
SDS-polyacrylamide electrophoresis and western blotting applied to the study of asthma / Virginia García-Solaesa and Sara Ciria Abad --
Chromatin immunoprecipitation : application to the study of asthma / Asunción García-Sánchez and Fernándo Marqués-García --
Gene silencing delivery methods : lipid-mediated and electroporation transfection protocols / Asunción García-Sánchez and Fernando Marqués-García --
Protocols for exosome isolation and RNA profiling / Fernando Marqués-García and María Isidoro-García --
Cell culture techniques : corticosteroid treatment in A549 human lung epithelial cell / Elena Marcos-Vadillo and Asunción García-Sánchez --
Protocol for lipid-mediated transient transfection in A549 epithelial lung cell line / Elena Marcos-Vadillo and Asunción García-Sánchez --
Promoter assay using luciferase reporter gene in the A549 cell line / Elena Marcos-Vadillo and Asunción García-Sánchez --
Review of mouse models applied to the study of asthma / Fernando Marqués-García and Elena Marcos-Vadillo --
General and specific mouse models for asthma research / Fernando Marqués-García and Elena Marcos-Vadillo --
Mouse models applied to the research of pharmacological treatments in asthma / Fernando Marqués-García and Elena Marcos-Vadillo --
Review on pharmacogenetics and pharmacogenomics applied to the study of asthma / Almudena Sánchez-Martín, Asunción García-Sánchez, and María Isidoro-García.
Series Title: Methods in molecular biology (Clifton, N.J.), 1434.
Responsibility: edited by María Isidoro-García.
More information:

Abstract:

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.

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