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Molecular microbiology : diagnostic principles and practice

Author: David H Persing
Publisher: Washington, DC : ASM Press, ©2011.
Series: Reference Manual, ASM Press.
Edition/Format:   eBook : Document : English : 2nd ed
Summary:
The second edition presents the latest molecular diagnostic techniques to support clinical care and basic and clinical research. The authors all experienced researchers and diagnosticians have conducted a comprehensive review and evaluation of this rapidly evolving field and provide the new material in an easy-to-read summary format. Moreover, the book offers a broad range of practical advice, such as determining  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Molecular microbiology.
Washington, DC : ASM Press, ©2011
(DLC) 2010023700
(OCoLC)640072404
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: David H Persing
ISBN: 9781555816834 1555816835
OCLC Number: 711040513
Description: 1 online resource (xvi, 936 pages) : illustrations (some color).
Contents: Diagnostic Principles --
I. Molecular Hybridization for identification --
1 Applications of Fluorescence In Situ Hybridization in Diagnostic Microbiology / Stefan Juretschko and Thomas R. Fritsche --
2. DNA Probes for Culture Confirmation and Direct Detection of Bacterial and Fungal Infections : a Review of Current Technologies and Assays / Julie Kingery and Karen C. Carroll --
II. Nucleic Acid Amplification Methods --
3. In Vitro Nucleic Acid Amplification Techniques / Vivekanand Datta and Randall T. Hayden --
4. Real-Time PCR and Melting Analysis / Carl T. Wittwer and Noriko Kusukawa --
5. Quantitative Molecular Methods / Donna M. Wolk and Randall T. Hayden --
6. Use of Commercial Amplification Tests in the Clinical Microbiology Laboratory : Test Selection and Quality Assurance / Gerri S. Hall and et al. --
III. Laboratory operations --
7. Nucleic Acid Isolation : Overview of Sample Preparation Methods / Charles E. Hill --
8. Laboratory Design and Operations / P. Shawn Mitchell, Jeffrey J. Germer, and Joseph D.C. Yao --
IV. Molecular Typing Methods --
9. Molecular Phylogenetic Analysis / Les Dethlefsen, Paul W. Lepp, and David A. Relman --
10. Pulsed-Field Gel Electrophoresis : Laboratory and Epidemiologic Considerations for Interpretation of Data / Richard V. Goering, Efrain M. Ribot, and Peter Gerner-Smidt --
11. Strain Typing Using Multiple "Variable Number of Tandem Repeat" Analysis and Genetic Element CRISPR / Christine Pourcel and Gilles Vergnaud --
12. Repetitive Sequence-Based PCR Typing of Bacteria and Fungi / Stacie R. Frye and Mimi Healy --
13. Molecular Typing Methods for Analysis of Extraintestinal Pathogenic Escherichia coli / James R. Johnson --
V. Detection Methodology --
14. Fluorescence Resonance Energy Transfer / J.R. Uhl, Y.W. Tang, and F.R. Cockerill III --
15. Detection of Pathogenic Organisms with Multicolor Molecular Beacons / Fann Wu, and et al. --
16. Microwell Plate Detection Systems for Amplicon Detection and Characterization / Jiang Fan and Michael J. Loeffelholz --
17. Detection Methodology : Pyrosequencing / Liying Yan and Robert Slinger --
18. Solid- and Liquid-Phase Array Technologies / Melissa B. Miller --
VI. New Technologies --
19. Next-Generation DNA Sequencing and Microbiology / Russell Higuchi, Ulf Gyllensten, and David H. Persing --
20. Raman Spectroscopy for Bacterial Strain Typing / Diana Willemse-Erix, Alex van Belkum, and Kees Maquelin --
21. Multiplex PCR Product Detection and Discrimination / Steven D. Zink, Nick M. Cirino, and Christina Egan --
22. Detection of Emerging Antimicrobial Resistance by Use of the Ibis T5000 Universal Biosensor / David J. ecker and et al. --
Diagnostic Applications --
VII. Molecular Detection of Bacterial Organisms --
23. Update on the Detection and Characterization of Bacterial Pathogens by Nucleic Acid Amplification / K. Loens, H. Goossens, and M. Ieven --
24. Molecular Detection of Chlamydia trachomatis and Neisseria gonorrhoeae / Jens K. Moller and et al. --
25. PCR Detection of Haemophilus ducreyi, Treponema pallidum, and Mycoplasma genitalium / Patricia A. Totten, Lisa E. Manhart, and Arturo Centurion-Lara --
26. Molecular Detection and Characterization of Mycobacterium tuberculosis / Betty A. Forbes --
27. Sequence-Based Identification and Characterization of Mycobacteria / Nancy L. Wengenack and Leslie Hall --
28. Molecular Detection of Group B Streptococcus / Preeti Pancholi --
29. Molecular Detection and Identification of Methicillin-Resistant Staphylococcus aureus / Willem van Leeuwen and Alex van Belkum --
30. Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory / Rosemary C. She, Keith E. Simmon, and Cathy A. Petti --
31. Broad-Range PCR for Detection and Identification of Bacteria / Matthias Maiwald --
32. Detection of Antimicrobial Resistance Genes and Mutations Associated with Antimicrobial Resistance in Bacteria / Fred C. Tenover and J. Kamile Rasheed --
33. Detection of Agents Associated with Bioterrorism / Paul Keim, Victor Waddell, and David M. Engelthaler --
VIII. Molecular Detection and Characterization of Viruses --
34. Diagnostic Molecular Virology : Current Practice and Future Trends / Frederick S. Nolte --
35. Molecular Detection and Characterization of HIV-1 / Angela M. Caliendo and Colleen S. Kraft --
36. Molecular Detection and Characterization of Hepatitis C Virus / Michael S. Forman and Alexandra Valsamakis --
37. Molecular Detection and Characterization of Hepatitis B Virus / David R. Hillyard --
38. Molecular Detection of Human Papillomaviruses / Denise I. Quigley and Elizabeth R. Unger --
39. Molecular Detection of Respiratory Viruses / Richard S. Buller and Max Q. Arens --
40. Viral Infections in Transplant Recipients / Robin Patel and Frederick S. Nolte --
41. New Virus Discovery in the 21st Century / Stacy Finkbeiner and David Wang --
IX. Detection and Characterization of Fungal pathogens --
42. Molecular Detection and Characterization of Fungal Pathogens / Stephane Bretagne --
43. Sequence-Based Fungal Identification and Classification / Jianli Dong, Michael J. Loeffelholz, and Michael R. McGinnis --
44. Molecular Detection of Antifungal Resistance / Thomas D. Edlind --
X. Detection and Characterization of Parasitic Protozoa --
45. Overview of the Development, Utility, and Future of Molecular Diagnostics for Parasitic Diseases / Bobbi Pritt and Jon Rosenblatt --
46. Molecular Approaches for Diagnosis of Malaria and Characterization of Genetic Markers of Drug Resistance / Laura K. Erdman, Michael Hawkes, and Kevin C. Kain --
47. Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi / Michal Svoboda and et al. --
XI. Systems Microbiology --
48. Molecular Diagnosis of Gastrointestinal Infections / Benjamin A. Pinsky and Niaz Banaei --
49. Molecular Approaches to the Diagnosis of Sepsis / Paul H.M. Savelkoul and Remco P.H. Peters --
50. Molecular Approaches to the Diagnosis of Meningitis and Encephalitis / Karen C. Bloch and Yi-Wei Tang --
XII. The Pathogen/Host Interface --
51. Genomics and DNA Variation : Determinants of Susceptibility and Outcomes in Microbial Diseases / Chiea-Chuen Khor, Stephen J. Chapman, and Adrian V.S. Hill --
52. Genetically Polymorphic Cytochrome P450s and Transporters and Personalized Antimicrobial Chemotherapy / Hong-Guang Xie --
53. Host Genomic Profiling in Human Immunodeficiency Virus Infection / Kevin V. Shianna and Amalio Telenti --
54. Innate Immunity and Host Defense against Microbial Infection / Markus Schnare and Salman Qureshi --
XIII. Laboratory Standardization, Proficiency-Testing Programs, Quality-Control Standards, and Monitoring --
55. Molecular Method Verification / Donna M. Wolk and Elizabeth M. Marlowe --
56. Molecular Test Validation, Monitoring, and Quality Control / Matthew J. Bankowski --
57. External Quality Assessment and Proficiency Testing in Diagnostic Molecular Microbiology / Michael J. Mitchell --
58. Laboratory Controls and Standards / Maurice Exner.
Series Title: Reference Manual, ASM Press.
Responsibility: edited by David H. Persing [and 5 others].

Abstract:

provides complete coverage of the principles and applications of molecular microbiology as they pertain to infectious diseases; summarizes the basic scientific theory underlying molecular  Read more...

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    schema:description "Diagnostic Principles -- I. Molecular Hybridization for identification -- 1 Applications of Fluorescence In Situ Hybridization in Diagnostic Microbiology / Stefan Juretschko and Thomas R. Fritsche -- 2. DNA Probes for Culture Confirmation and Direct Detection of Bacterial and Fungal Infections : a Review of Current Technologies and Assays / Julie Kingery and Karen C. Carroll -- II. Nucleic Acid Amplification Methods -- 3. In Vitro Nucleic Acid Amplification Techniques / Vivekanand Datta and Randall T. Hayden -- 4. Real-Time PCR and Melting Analysis / Carl T. Wittwer and Noriko Kusukawa -- 5. Quantitative Molecular Methods / Donna M. Wolk and Randall T. Hayden -- 6. Use of Commercial Amplification Tests in the Clinical Microbiology Laboratory : Test Selection and Quality Assurance / Gerri S. Hall and et al. -- III. Laboratory operations -- 7. Nucleic Acid Isolation : Overview of Sample Preparation Methods / Charles E. Hill -- 8. 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