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Monoclonal Antibodies : Hybridomas: A New Dimension in Biological Analyses

Author: Roger H Kennett; Thomas J McKearn; Kathleen B Bechtol
Publisher: Boston, MA : Springer US, 1980.
Edition/Format:   eBook : Document : EnglishView all editions and formats

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Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Roger H Kennett; Thomas J McKearn; Kathleen B Bechtol
ISBN: 9781461575054 1461575052 9781461575078 1461575079
OCLC Number: 840286381
Description: 1 online resource
Contents: I Introduction Production of Specific Antibodies from Continuous Cell Lines in Vitro.- 1 Plasmacytomas and Hybridomas Development and Applications.- I. Introduction.- II. Experimental Uses of Plasmacytomas.- III. The Fusion of Mouse Myeloma to Immune Spleen Cells.- IV. Applications of Monoclonal Antibodies.- V. The Use of Cell Fusion to Obtain Functional Cell Lines.- VI. Conclusions.- References.- 2 Continuously Proliferating Human Cell Lines Synthesizing Antibody of Predetermined Specificity.- I. Introduction.- II. Specific Antibody-Producing Cell Lines.- III. Preselection of Antibody-Producing Cells.- IV. In Vitro Immunization of Human B Lymphocytes.- V. Conclusions.- References.- II Analysis of Immunoglobulin Structure and Genetics.- 3 Antibody Diversity Patterns and Structure of Idiotypic Determinants on Murine Anti- ?(1 ? 3) Dextran Antibodies.- I. Introduction.- II. Mouse Anti-?(1 ? 3) Dextran Serum Antibodies.- III. Monoclonal Antidextran Antibodies from Somatic Cell Hybrids.- IV. Structure of VH Regions from Antidextran Antibodies.- V. Structural Correlates of Idiotypic Determinants.- VI. Implications from These Studies.- VII. Discussion.- VIII. Summary.- References.- 4 Defining the B-Cell Repertoire with Hybridomas Derived from Monoclonal Fragment Cultures.- I. Introduction.- II. The Use of Hybridomas in Defining the B-Cell Repertoire.- III. The Neonatal B-Cell Repertoire.- IV. Hybridomas Obtained from Neonatal Splenic Fragment Cultures.- References.- 5 The Use of Hybridomas to Localize Mouse Immunoglobulin Genes.- I. Introduction.- II. The Experimental System.- III. Discussion.- References.- III Detection and Analysis of Human Gene Products.- 6 Monoclonal Antibodies as Tools for Human Genetic Analysis.- I. Introduction.- II. Monoclonal Antibodies against a Variety of Human Gene Products.- III. Somatic Cell Hybrids.- IV. Erythrocyte-Specific Antigens.- V. Thymocyte-Specific Antigen.- VI. Discussion.- VII. Conclusion.- References.- 7 The Use of Hybridomas in Enzyme Genetics.- I. Introduction.- II. Methodology for Production and Detection of Antibodies against Human Placental Alkaline Phosphatase.- III. Results and Discussion.- IV. Concluding Remarks.- References.- 8 Characterization of a Human T-Cell Population Identified and Isolated by a Monoclonal Antibody.- I. Introduction.- II. Production and Selection of Antibodies to Human T Cells.- III. Characterization of a Human T-Cell Antigen Defined by a Monoclonal Antibody.- IV. Discussion.- References.- 9 Mouse x Human Hybridomas.- I. Introduction.- II. Mouse Myeloma Cells Hybridized with Human B-Cell Tumors.- III. Characterization of the Secreted Human Immunoglobulin.- IV. Relationship between Secreted ? Chains and Membrane ? Chains.- V. Relationship between Production of ? Chain and the Presence of ? Chain mRNA.- VI. Production of an Antiidiotype Reagent against the Rescued Human Immunoglobulin.- VII. Summary.- References.- 10 Monoclonal Antibodies against Human Tumor-Associated Antigens.- I. Introduction.- II. Tumor-Specific Antigens and Tumor-Associated Antigens.- III. Human Tumor Antigens-Detection by Monoclonal Antibodies.- IV. Antibodies to Human Neuroblastoma.- V. Detection of Metastatic Neuroblastoma.- VI. Monoclonal Antibodies and Genetics of Human Tumor Antigens.- References.- IV Monoclonal Antibodies as Probes in the Study of Cellular Differentiation and Immunogenetics.- 11 Germ-Cell-Related and Nervous-System-Related Differentiation and Tumor Antigens.- I. Introduction.- II. Immunizations.- III. Use of Monoclonal Antibodies to Study Spermatogenesis.- IV. Use of Monoclonal Antibodies to Study Differentiation and Tumors of the Nervous System.- V. Reactivity of Anti-Testis and Anti-C1300 Hybridomas.- VI. Conclusion.- References.- 12 Cell-Surface Differentiation in the Mouse Characterization of "Jumping" and "Lineage" Antigens Using Xenogeneic Rat Monoclonal Antibodies.- I. Introduction.- II. Techniques for Obtaining and Characterizing Monoclonal Antibodies to Differentiation Antigens.- III. Properties of Rat Monoclonal Antibodies.- IV. Murine Differentiation Antigens Identified by Rat Monoclonal Antibodies.- V. Patterns of Differentiation Antigen Expression.- VI. Concluding Remarks.- References.- 13 Rat-Mouse Hybridomas and Their Application to Studies of the Major Histocompatibility Complex.- I. Introduction.- II. General Properties of Rat-Mouse Hybridomas.- III. Application of Hybridoma Technology to Studies of the Rat Major Histocompatibility Complex.- References.- 14 Murine T-Cell Differentiation Antigens Detected by Monoclonal Antibodies.- I. Introduction.- II. Detection and Analysis of Monoclonal Antibodies Reactive with Cell-Surface Antigens.- III. Two Antigenic Specificities of Mouse Thy-1 Recognized by Monoclonal Antibodies.- IV. Characterization of Lyt-1 and Lyt-2 Glycoproteins with Monoclonal Antibodies.- V. Identification of Two Previously Unknown T-Cell Differentiation Antigens.- References.- 15 Monoclonal Antibodies That Define T-Lymphocyte Subsets in the Rat.- I. Introduction.- II. Production and Detection of Antibodies.- III. Labeling of Rat Lymphoid Cells with the Monoclonal Antibodies.- IV. Amount of Antigen per Cell and Expression on Other Tissues.- V. Functional Studies.- VI. Inhibition of the Mixed Lymphocyte Reactions by W3/25 Antibody.- VII. Discussion.- VIII. Summary.- References.- 16 Monoclonal Antibody Therapy of Mouse Leukemia.- I. Introduction.- II. Characterization of Anti-Thy-1 Antibodies Used for Serum Therapy.- III. Influence of Monoclonal Anti-Thy-1.1 Antibody on Murine Leukemia Cells.- IV. Pharmacokinetics of Monoclonal Antibodies.- V. Combined Surgical and Antibody/Complement Treatment.- VI. Mechanism of Antitumor Effects in Vivo.- VII. Influence of Monoclonal Antibody on Normal T Cells in Vivo.- VIII. Concluding Remarks.- References.- V Monoclonal Antibodies to Microorganisms.- 17 Mapping of Viral Proteins with Monoclonal Antibodies Analysis of the Envelope Proteins of Murine Leukemia Viruses.- I. Introduction.- II. Isolation of Hybrid Cell Lines Producing Monoclonal Antiviral Antibodies.- III. Serological Characteristics of Antiviral Monoclonal Antibodies.- IV. Mapping of Viral Proteins with Monoclonal Antibodies.- References.- 18 Monoclonal Antibodies against Influenza Virus.- I. Introduction.- II. The Generation of a Diverse Panel of Antiinfluenza Hybridomas.- III. Study of Viral Antigens.- IV. Study of the Antiviral Immune Response.- V. Appendix.- References.- 19 Monoclonal Antibodies against Rabies Virus.- I. Introduction.- II. Monoclonal Antibodies against Rabies Virus.- III. Selection of Rabies Virus Variants.- IV. Protection of Mice by Hybridoma Antibody.- V. Conclusions.- References.- 20 Monoclonal Antibodies against Streptococcal Antigens.- I. Importance of Group B Streptococci as a Pathogen in Neonates.- II. Production of Monoclonal Antibodies against Type II and Type.- Group B Streptococcus.- III. Discussion.- References.- Appendix Methods for Production and Characterization of Monoclonal Antibodies.- Plasmacytoma Cell Lines.- Fusion by Centrifugation of Cells Suspended in Polyethylene Glycol.- Roger H. Kennett.- Fusion of Cells in an Adherent Monolayer.- Thomas J. McKearn.- Fusion of Cells in Suspension and Outgrowth of Hybrids in Conditioned Medium.- Walter Gerhard.- Cloning of Hybridomas Cloning in Semisolid Agarose.- Roger H. Kennett.- Cloning of Hybridoma Cells by Limiting Dilution in Fluid Phase.- Thomas J. McKearn.- Freezing of Hybridoma Cells.- Roger H. Kennett.- Enzyme-Linked Antibody Assay with Cells Attached to Polyvinyl Chloride Plates.- Roger H. Kennett.- Peroxidase-Conjugated Antiglobulin Method for Visual Detection of Cell-Surface Antigens.- Zdenka L. Jonak.- Radioimmunoassay.- Kathleen B. Bechtol.- Quantitative Absorption/Blocking Assay.- Kathleen B. Bechtol.- Radioimmunoassay for Genetic Screening of Proteins Using Monoclonal Antibodies.- C. A. Slaughter, M. C. Coseo, C. Abrams, M. P. Cancro, and H. Harris.- Binding of Hybridoma Antibodies to Polyvinyl Chloride Microtiter Dishes.- Thomas J. McKearn.- Microcytotoxicity Assay.- Roger H. Kennett.- Thomas J. McKearn.- Immunoprecipitation with Monoclonal Antibodies.- Lois A. Lampson.- Binding Inhibition Using Biosynthetically Labeled Monoclonal Antibody.- Lois A. Lampson.- Protein lodination Suitable for Labeling Hybridoma Antibodies.- Dennis M. Klinman and J. C. Howard.- Method for Growing Hybridomas in Rats or Mice.- Thomas J. McKearn.- Zdenka L. Jonak.- Characterization of Hybridoma Immunoglobulins by Sodium Dodecylsulf ate-Polyacrylamide Gel Electrophoresis.- J. B. Haas and Roger H. Kennett.- The Calculation of Antigen-Antibody Binding Constants by Radioimmunoassay.- Mark E. Frankel.- References to Other Methods.
Responsibility: edited by Roger H. Kennett, Thomas J. McKearn, Kathleen B. Bechtol.


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