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Mucins : methods and protocols

Author: Michael A McGuckin; David J Thornton
Publisher: New York : Humana : Springer, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 842.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing  Read more...
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Details

Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Mucins.
New York : Humana ; Springer, ©2012
(OCoLC)752068546
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Michael A McGuckin; David J Thornton
ISBN: 9781617795138 1617795135
OCLC Number: 773842685
Language Note: English.
Description: 1 online resource (xi, 329 pages) : illustrations.
Contents: Mucin methods : genes encoding mucins and their genetic variation with a focus on gel-forming mucins / Karine Rousseau and Dallas M. Swallow --
Gel-forming and cell-associated mucins : preparation for structural and functional studies / Julia R. Davies, Claes Wickstrom, and David J. Thornton --
Detecting, visualising, and quantifying mucins / Ceri A. Harrop, David J. Thornton, and Michael A. McGuckin --
Mass spectrometric analysis of mucin core proteins / Mehmet Kesimer and John K. Sheehan --
O-glycoprotein biosynthesis : site localization by edman degradation and site prediction based on random peptide substrates / Thomas A. Gerken --
Analysis of assembly of secreted mucins / Malin E.V. Johansson and Gunnar C. Hansson --
MUC1 membrane trafficking : protocols for assessing biosynthetic delivery, endocytosis, recycling, and release through exosomes / Franz-Georg Hanisch [and others] --
Glycomic work-flow for analysis of mucin o-linked Oligosaccharides / Catherine A. Hayes [and others] --
O-glycomics : profiling and structural analysis of mucin-type o-linked glycans / Isabelle Breloy --
O-glycoproteomics : site-specific o-glycoprotein analysis by CID/ETD electrospray ionization tandem mass spectrometry and top-down glycoprotein sequencing by in-source decay MALDI mass spectrometry / Franz-Georg Hanisch --
Analysing mucin degradation / Stephen D. Carrington [and others] --
Assessment of mucus thickness and production in situ / Lena Holm and Mia Phillipson --
Preservation of mucus in histological sections, immunostaining of mucins in fixed tissue, and localization of bacteria with FISH / Malin E.V. Johansson and Gunnar C. Hansson --
Ex vivo measurements of mucus secretion by colon explants / Jenny K. Gustafsson, Henrik Sjovall, and Gunnar C. Hansson --
Establishment of respiratory air-liquid interface cultures and their use in studying mucin production, secretion and function / David B. Hill and Brian Button --
Studying mucin secretion from human bronchial epithelial cell primary cultures / Lubna H. Abdullah [and others] --
Assessment of intracellular mucin content in vivo / Lucia Piccotti, Burton F. Dickey, and Christopher M. Evans --
Techniques for assessment of interactions of mucins with microbes and parasites in vitro and in vivo / Yong H. Sheng [and others] --
Assessing mucin expression and function in human ocular surface epithelia in vivo and in vitro / Pablo Argueso and Ilene K. Gipson.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 842.
Responsibility: edited by Michael A. McGuckin, David J. Thornton.

Abstract:

This volume presents an overview of the methods commonly used to study Mucins. These include the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics, in  Read more...

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