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Mycoplasma protocols

Author: Roger Miles; Robin Nicholas
Publisher: Totowa, N.J. : Humana Press, 1998.
Series: Methods in molecular biology (Clifton, N.J.), v. 104.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp.

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Genre/Form: Laboratory Manuals
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Mycoplasma protocols.
Totowa, N.J. : Humana Press, 1998
(DLC) 98004288
(OCoLC)38976151
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Roger Miles; Robin Nicholas
ISBN: 0585328552 9780585328553 9781592592692 1592592694 0896035255 9780896035256
OCLC Number: 45842675
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xiii, 330 pages).
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Medical significance of mycoplasmas / Paul Taylor --
The veterinary significance of mycoplasmas / Robin Nicholas --
Recovery of human mycoplasmas / Paul Taylor --
Recovery of mycoplasmas from animals / Robin Nicholas, Samantha Baker --
Recovery of mycoplasmas from birds / Janet M. Bradbury --
Cultivation of ureaplasmas / Patricia M. Furr --
Quality-control testing of mycoplasma medium / David Windsor, Helena Windsor --
Biochemical characteristics in mycoplasma identification / José B. Poveda --
Enzyme analysis : the rationale and use of enzyme assays in assigning function to gene nucleotide sequences and the procedures for the assay of three enzymatic functions conserved in mollicutes / J. Dennis Pollack --
Determination of substrate utilization rates by mycoplasmas / Roger J. Miles, Calista Agbanyim --
Serological identification of mycoplasmas by growth and metabolic inhibition tests / José B. Poveda, Robin Nicholas --
Identification of mycoplasmas by dot immunobinding on membrane filtration (MF Dot) / Francois Poumarat --
Identification of mycoplasmas by immunofluorescence / Janet M. Bradbury --
Diagnostic application of monoclonal antibody (MAb)-based sandwich ELISAs / Hywel J. Ball, David Finlay --
Immunohistochemical staining of fixed tissues / Eugenio Scanziani --
Extraction of DNA from mycoplasmas / John B. Bashiruddin --
Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers / Karl-Erik Johansson, Malin U. Heldtander, Bertil Pettersson --
PCR and RFLP methods for the specific detection and identification of Mycoplasma mycoides subsp. mycoides SC / John B. Bashiruddin --
Characterization of mycoplasmas by RAPD fingerprinting / Georges A. Rawadi --
Classification of isolates by DNA-DNA hybridization / Konrad Sachse, Helmut Hotzel --
Insertion sequence analysis / Joachim Frey --
Detection of mycoplasmas in cell cultures by cultural methods / Gerald K. Masover, Frances A. Becker --
Detection of mycoplasmas in cell cultures by fluorescence methods / Gerald K. Masover, Frances A. Becker --
Transformation of mycoplasmas / Chris F. Minion, Paul A. Kapke --
Transposon mutagenesis / LeRoy L. Voelker, Kevin Dybvig --
Demonstration of extrachromosomal elements / LeRoy L. Voelker, Kevin Dybvig --
Expression of foreign genes in Acholeplasma laidlawii / Tanja K Jarhede, Åke Wieslander --
Mycoplasma gene expression in Escherichia coli / Chris F. Minion --
Polyacrylamide gel-electrophoresis separation of whole-cell proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham --
Immunological and biochemical characterization of membrane proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham --
Detection and analysis of mycoplasma adhesins / Konrad Sachse --
Transmission electron microscopy and immunogold staining of mollicute surface antigens / Gunna Christiansen, Svend Birkelund.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 104.
Responsibility: edited by Roger Miles and Robin Nicholas.
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