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Neurodegeneration methods and protocols

Author: Jean Harry; Hugh A Tilson
Publisher: Totowa, N.J. : Humana Press, ©1999.
Series: Methods in molecular medicine, 22.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Rapid advances in our understanding of basic cell biological processes and of the molecular mechanisms of cell function and dysfunction have led to an increasing interest in utilizing these  Read more...

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Genre/Form: Fulltext
Internet Resources
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Jean Harry; Hugh A Tilson
ISBN: 089603612X 9780896036123
OCLC Number: 39982678
Description: xi, 306 pages : illustrations ; 24 cm.
Contents: Introduction to polymerase chain reaction / Mark C. Miller and Laura Cunningham --
Methodological issues in polymerase chain reaction for RNA / Alessandra D. Rasmussen --
Subtractive hybridization of cDNA libraries / Gregory S. Kelner and Richard A. Maki --
The use of differential display RT-PCR for identifying altered gene expression in ischemic brain injury / Xinkang Wang and Giora Z. Feuerstein --
Analysis of gene expression by multiprobe RNase protection assay / Anna K. Stalder [and others] --
Molecular probes for PNS neurotoxicity, degeneration, and regeneration / Arrel D. Toews --
Mobility-shift DNA-binding assay using gel electrophoresis / Yang Xiao and Keith Pennypacker --
Transfection of mammalian cells in vitro: use in analysis of neuronal damage / Melvin L. Billingsley and Theresa A. Thompson --
Detection of apoptotic cells in the vervous system --
Terrie Kagan and Zahra Zakeri --
Translocation assays of protein kinase C activation / Ginger G. Wescott, Christina M. Manring, and David M. Terrian --
Astrocyte and neuron coculturing method / Michael Aschner and Barbara A. Bennett --
Viability assays for cells invitro: the ethidium/calcium assay and the immunofluorescence combination assay / Theresa A. Thompson --
Measurement of the nitric oxide synthase activity using the citrulline assay / Thomas R. Ward and William R. Mundy --
Measurement of intraneuronal free calcium using the fluorescencent probe technique / Prasada Rao S. Kodavanti --
Measurement of calcium buffering by intracellular organelles in brain / Prasada Rao S. Kodavanti --
Receptor-mediated release of inositolphosphates in brain slices / Pushpa Tandon --
The use of enzyme-linked immunosorbent assays to quantitate proteins in biological functions / Dori R. Germolec --
Assays for the analysis of synaptic proteins in neurodegenerative disorders / Eliezer Masliah and Michael Alford --
ZZymographic method for the measurement of monoamine neurotransmitters in microdialysis perfusates using HPLC-ECD / Ian Acworth and Michael L. Cunningham --
Biochemical measurement of cholinesterase activity / Stephanie Padilla, T. Leon Lassiter, and Deborah Hunter --
Functional study of glutamate receptor channels in brain slices / Susan Jones and Jerrel L. Yakel --
Selective silver staining for neuronal degeneration / Jeanene K. Olin and Karl F. Jensen --
Immunohistological markers of cerebrovascular integrity / Karl F. Jensen [and others] --
Electron probe X-ray microanalysis: a quantitative electron microscopy technique for measurement of elements and water in nervous tissue cells / Richard M. LoPachin and Christopher L .Gaughan.
Series Title: Methods in molecular medicine, 22.
Responsibility: edited by Jean Harry and Hugh A. Tilson.
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". . .the authors are to be commended for the consistency in format and altruism shown contributors to represent the procedures with minimal self-serving recounting of their use of the method."-The Read more...

 
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