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Neurotrophin protocols

Author: Robert A Rush
Publisher: Totowa, NJ : Humana Press, ©2001.
Series: Methods in molecular biology (Clifton, N.J.), v. 169.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular  Read more...
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Genre/Form: Laboratory Manuals
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Neurotrophin protocols.
Totowa, NJ : Humana Press, ©2001
(DLC) 00044824
(OCoLC)44420495
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Robert A Rush
ISBN: 0896036995 9780896036994 9781592590605 1592590608
OCLC Number: 170167020
Description: 1 online resource (x, 274 pages) : illustrations.
Contents: Localization of neurotrophin proteins within the central nervous system by immunohistochemistry / J.M. Conner --
Neurotrophin immunohistochemistry in peripheral tissues / R.A. Rush and X.F. Zhou --
Extraction and quantification of the neurotrophins / S.H. Zhang, X.F. Zhou and R.A. Rush --
Identifying novel proteins in nervous tissue using microsequencing techniques / S.E. Bartlett --
Quantification of mRNA levels using northern blotting / P. Fernyhough --
Quantification of mRNA levels using ribonuclease protection assay / P. Henttu --
Quantification of neurotrophin mRNA by RT-PCR / E. Chie [and others] --
Sensitive and nonradioactive in situ detection of neurotrophin mRNAs in the nervous system / C.J. Xian and X.F. Zhou --
Expression of neurotrophic factors such as GDNF in the E. Coli system / B.R. Huang and X.M. Ma --
Construction of cells expressing neurotrophins / J. Ray and F.H. Gage --
Efficient generation of stable pheochromocytoma (PC12) cell lines using a recombinant retrovirus (lnc) / M.E. Cunningham, J.K. Kitajewski and L.A. Greene --
Construction and analysis of transgenic animals overexpressing neurotrophins / K.M. Albers and B.M. Davis --
Construction of transgenic animals overproducing neurotrophins and their receptors / C.G. Causing and F.D. Miller --
Tracing with radiolabeled neurotrophins / C.S. von Bartheld --
Toxin-induced death of neurotrophin-sensitive neurons / R.G. Wiley --
Oligonucleotides as inhibitors of protein synthesis / W.R. Epa, G.L. Barrett and P.F. Bartlett --
The IODO-GEN method for labeling and the use of retrograde axonal transport to assay neurotrophins / I.A. Hendry and A.J. Reynolds --
Stereological estimation of neuronal number. The optical disector/cavalieri combination / V. Nurcombe, N.G. Wreford and J.F. Bertram. Neurotrophin quantification and localization --
RNA quantification and localization --
Construction of recombinant proteins, and transgenic cells and animals --
In vivo analysis.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 169.
Responsibility: edited by Robert A. Rush.

Abstract:

The past decade has seen an extraordinary growth in research interest in neurotrophic factors, and the study of the neurotrophin family has led this activity. Neurotrophin Protocols has brought  Read more...

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"...provides a collection of chapters written by experts in developmental neuroscience, neuroanatomy, signaling mechanisms that regulate neuronal survival and plasticity, and neurodegenerative Read more...

 
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