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New protein techniques

Author: John M Walker
Publisher: Clifton, N.J. : Humana Press, ©1988.
Series: Methods in molecular biology (Clifton, N.J.), v. 3.; Biological methods.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Fulltext
Internet Resource
Electronic books
Additional Physical Format: Print version:
New protein techniques.
Clifton, N.J. : Humana Press, ©1988
(DLC) 92130040
(OCoLC)19018480
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: John M Walker
ISBN: 9781592594900 1592594905
OCLC Number: 234536920
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xv, 531 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Prevention of unwanted proteolysis / Robert J. Beynon --
The Bradford method for protein quantitation / John B.W. Hammond, Nicholas J. Kruger --
Amino acid analysis by precolumn derivatization ; Identification of N-terminal amino acids by high-performance liquid chromatography / E.L.V. Harris --
Enzymatic methods for cleaving proteins ; Chemical cleavage of proteins / Bryan John Smith --
Chemical modification of proteins / Alex F. Carne --
The design, preparation, and use of immunopurification reagents / Andrew Kenney, Lynne Goulding, Christopher Hill --
Dye-ligand chromatography / Sarojani Angal --
Aminohexyl-Sepharose affinity chromatography : purification of an auxin receptor / R.D.J. Barker, H.M. Bailey --
Purification of DNA-dependent RNA polymerase from eubacteria / N.W. Scott --
Direct immunoprecipitation of protein / Christopher F. Thurston, Lucy F. Henley --
Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique / Ketan Patel, David J. Easty, Michael J. Dunn --
Chromatofocusing / Ake Sidén, Paolo Gallo --
Hybrid isoelectric focusing using mixed synthetic-carrier ampholyte-immobilized pH gradient gels ; Two-dimensional electrophoresis using immobilized pH gradients in the first dimension ; Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension / Michael J. Dunn, Ketan Patel --
Preparative aspects of immobilized pH gradients / Pier Giorgio Righetti, Cecilia Gelfi --
Isoelectric focusing under denaturing conditions / Christopher F. Thurston, Lucy F. Henley --
Computer analysis of 2-D electrophoresis gels : image analysis, data base, and graphic aids / H. H-S. Ip --
Two-dimensional (crossed) immunoelectrophoresis / John M. Walker --
Peptide synthesis ; Synthesis of a series of analogous peptides using T-bags / Brian Austen --
Production of antisera to synthetic peptides / W.J. Gullick --
Production of antibodies using protein in gel bands / S.A. Amero, T.C. James, S.C.R. Elgin --
Purification of immunoglobulins using protein A-Sepharose / Nicholas J. Kruger, John B.W. Hammond --
Antibody-enzyme conjugate formation / G.B. Wisdom --
Vacuum blotting : an inexpensive, flexible, qualitative blotting technique ; Blotting with plate electrodes / Marnix Peferoen --
Use of dried milk for immunoblotting / Rosemary Jagus, Jeffrey W. Pollard --
Immunodetection of proteins on "Western" blots using ¹²⁵I-labeled protein A / Nicholas J. Kruger, John B.W. Hammond --
Detection of protein blots using the avidin-biotin system / Michael J. Dunn, Ketan Patel --
Detection of protein blots using enzyme-linked second antibodies or protein A / J.M. Walker, W. Gaastra --
Colloidal gold for the detection of proteins on blots and immunoblots / Alan Jones, Marc Moeremans --
Enzyme immobilization by adsorption ; Enzyme immobilization by entrapment ; Enzyme immobilization by covalent bonding ; Cell immobilization / M.D. Trevan.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 3.; Biological methods.
Responsibility: edited by John M. Walker.

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