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Nitric oxide : methods and protocols

Author: Helen O McCarthy; Jonathan A Coulter
Publisher: New York, N.Y. : Humana Press, ©2011.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 704.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a  Read more...
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Genre/Form: Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Nitric oxide.
New York, N.Y. : Humana Press, ©2011
(OCoLC)694534402
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Helen O McCarthy; Jonathan A Coulter
ISBN: 9781617379642 1617379646
OCLC Number: 700048823
Description: 1 online resource (xi, 226 pages) : illustrations.
Contents: Nitric oxide physiology and pathology --
Ionizing radiation-induced DNA strand breaks and [gamma]-H2AX[gamma]-H2AX foci in cells exposed to nitric oxide --
Determination of S-nitrosothiolsS-nitrosothiols in biological fluids by chemiluminescencechemiluminescence --
Measurement of nitritenitrite in blood samples using the ferricyanide-based hemoglobin oxidationoxidation assay --
Selective fluorescent activation for bioimagingbioimaging the expression of nitric oxide in cellular and in vivo systems --
Real-time measurement of murine hippocampushippocampus NO levels in response to cerebral ischemia/reperfusionreperfusion --
Detection of low levels of nitric oxide using an electrochemical sensor --
Determination of the scavenging capacity against reactive nitrogen species by automatic flow injection-based methodologies --
Aqueous measurement of nitric oxide using membrane inlet membrane inlet mass spectrometry --
Quantum cascade laser technology for the ultrasensitive detection of low-level nitric oxide --
Determination of in vivo nitric oxide levels in animal tissues using a novel spin trapping technology --
[Beta] cell protection by inhibition of iNOS through lentiviral vector-based strategies --
Characterization of nitric oxide delivery systems produced by various nanotechnologies --
Nitric oxide releasing nanoparticle synthesis and characterization --
NOS antagonism using viral vectors as an experimental strategy : implications for in vivo studies of cardiovascular control and peripheral neuropathies.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 704.
Responsibility: edited by Helen O. McCarthy, Jonathan A. Coulter.

Abstract:

Authoritative and easy to use, Nitric Oxide provides a detailed reference manual packed with step-by-step approaches to various complex protocols. The text examines advanced methodologies and  Read more...

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