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NMDA receptor protocols

Author: Min Li
Publisher: Totowa, N.J. : Humana Press, ©1999.
Series: Methods in molecular biology (Clifton, N.J.), v. 128.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
In NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
NMDA receptor protocols.
Totowa, N.J. : Humana Press, ©1999
(DLC) 98053445
(OCoLC)40453084
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Min Li
ISBN: 9781592596836 1592596835
OCLC Number: 170932237
Description: 1 online resource (x, 196 pages) : illustrations.
Contents: Isolation of receptor clones by expression screening in xenopus oocytes / F. Nakamura [and others] --
Expression of nmda receptor channel subunit proteins using baculovirus and herpesvirus vectors / S. Kawamoto [and others] --
Transient expression of functional nmda receptors in mammalian cells / P.L. Chazot, M. Cik and F.A. Stephenson --
Stable expression of human nmda receptors in cultured mammalian cells / M.A. Varney [and others] --
Expression of extracellular n-terminal domain of nmda receptor in mammalian cells / E.A. Whitehorn, W.J. Dower and M. Li --
Immunocytochemistry of nmda receptors / R.S. Petralia and R.J. Wenthold --
The use of ligand binding in assays of nmda receptor function / I.J. Reynolds and T.A. Sharma --
Calmodulin modification of nmda receptors / S. Zhang and R.L. Huganir --
Detergent solubilization and immunoprecipitation of native nmda receptors / R.J. Wenthold [and others] --
Redox sensitivity of nmda receptors / S.A. Lipton --
In vitro selection of peptides acting on nmda receptors / S.E. Cwirla, W.J. Dower and M. Li --
Nmda receptor pharmacology and analysis of patch-clamp recordings / J.M. Wright and R.W. Peoples --
Determination of membrane topology of glutamate receptors / H.M. VanDongen and A.M. VanDongen --
Engineering the nmda receptor channel lining / A.V. Ferrer-Montiel and M. Montal --
High-throughput functional detection of nmda receptor activity / M.D. Cunningham and S.M. Cunningham.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 128.
Responsibility: edited by Min Li.

Abstract:

Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection.  Read more...

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"...In this volume of Methods in Molecular Biology great care has been taken to present the most modern approaches to solving the mysteries of the NMDA receptor...Each chapter includes detailed Read more...

 
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