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Nonisotopic probing, blotting, and sequencing

Author: Larry J Kricka
Publisher: San Diego : Academic Press, ©1995.
Edition/Format:   eBook : Document : English : 2nd edView all editions and formats
Summary:
Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Nonisotopic probing, blotting, and sequencing.
San Diego : Academic Press, ©1995
(DLC) 94024916
(OCoLC)31328316
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Larry J Kricka
ISBN: 9780124262911 0124262910 9780080537665 0080537669
OCLC Number: 162129253
Description: 1 online resource (xvii, 518 pages, [2] pages of plates) : illustrations (some color)
Contents: Labels, Labelling, Analytical Strategies and Applications. Nonradioactive Labelling Methods for Nucleic Acids. Detection of Alkaline Phosphatase Labels By Time-Resolved Fluorescence. Detection of Alkaline Phosphatase By Bioluminescense. Detection of DNA on Membranes with Alkaline Phosphatase Labelled Probes and Chemiluminescence Cspdr Substrate. Detection of Alkaline Phosphatase By Colorimetry. Detection of Alkaline Phosphatase By Chemiluminescence Using NADP, Ascorbate and Indoxyl Phosphates. Detection of Horseradish Peroxidase By Enhanced Chemiluminescence. Detection of Horseradish Peroxidase By Colorimetry. Detection of Glucose 6-Phosphate Dehydrogenase By Bioluminescence. Detection of Xanthine Oxidase By Enhhanced Chemiluminescence. Electrochemiluminescence Detection of PCR-Derived Nucleic Acids. Detection of Phosphors By Phosphorescence. Detection of Europium Cryptates By Fluorescence. Detection of Lanthanid Chelates By Time-Resolved Fluorescence. Detection of Lanthanide Chelates and Multiple Labelling Strategies Based on Time-Resolved Fluorescence. Detection of Acridinium Esters By Chemiluminescence. Detection of Energy Transfer and Fluorescence Quenching. Sequencing By Nonisotopic Methods. Chemiluminesence DNA Sequencing with 1, 2-Dioxetanes. pt. 1. Introduction : Labels, labeling, analytical strategies, and applications --
Methods for nonradioactive labeling of nucleic acids. pt. 2. Detection methods : Detection of alkaline phosphatase labels by time-resolved fluorescence --
Detection of alkaline phosphatase by bioluminescence --
Detection of DNA on membranes with alkaline phosphatase-labeled probes and chemiluminescent CSPD[superscript R]substrate --
Detection of alkaline phosphatase by colorimetry --
Detection of alkaline phosphatase by chemiluminescence using NADP, ascorbate, and indolyl phosphates --
Detection of horseradish peroxidase by enhanced chemiluminescence --
Detection of horseradish peroxidase by colorimetry --
Detection of glucose-6-phosphate dehydrogenase by bioluminescence --
Detection of xanthine oxidase by enhanced chemiluminescence --
Electrochemiluminescent detection of PCR-derived nucleic acids --
Detection of phosphors by phosphorescence --
Detection of europium cryptates by time-resolved fluorescence --
Detection of lanthanide chelates by time-resolved fluorescence --
Detection of lanthanide chelates and multiple labeling strategies based on time-resolved fluorescence --
Detection of acridinium esters by chemiluminescence --
Detection of energy transfer and fluorescence quenching. pt. 3. DNA sequencing : DNA sequencing by nonisotopic methods --
Chemiluminescent DNA sequencing with 1,2-dioxetanes.
Responsibility: edited by Larry J. Kricka.
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Abstract:

Includes the improvements in nonisotopic tagging techniques for macromolecules to enable researchers to select the best nonisotopic method for their needs and maximize success by following its  Read more...

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