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Opioid research : methods and protocols

Author: Zhizhong Z Pan
Publisher: Totowa, N.J. : Humana Press, ©2003.
Series: Methods in molecular medicine, 84.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Opioid research has grown enormously over the last decade, and with it, the need to apply multiple techniques from a variety of disciplines. In Opioid Research: Methods and Protocols, expert researchers and physicians provide a comprehensive collection of the major cross-disciplinary methods and protocols used in current opioid research, covering topics from molecular and genetic techniques, to behavioral analyses  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Opioid research.
Totowa, N.J. : Humana Press, ©2003
(DLC) 2002192240
(OCoLC)51009166
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Zhizhong Z Pan
ISBN: 9781592593798 1592593798
OCLC Number: 171221714
Description: 1 online resource (x, 307 pages) : illustrations.
Contents: Molecular cloning of opioid receptors by cDNA library screening / Y.X. Pan --
Expression of opioid receptors in mammalian cell lines / Y.X. Pan --
Assessing opioid regulation of adenylyl cyclase activity in intact cells / D.R. Thakker, H.Z. Ozsoy and K.M. Standifer --
Analysis of opioid-induced kinase activation / L. Ma --
Study of opioid receptor phosphorylation using cell-labeling method with 32P-orthrophosphate / J.B. Wang --
Opioid receptor coupling to GIRK channels. In vitro studies using a Xenopus oocyte expression system and in vivo studies on weaver mutant mice / K. Ikeda [and others] --
Identification of alternatively spliced variants from opioid receptor genes / Y.X. Pan --
Immunohistochemical localization of mu-, delta- and kappa-opioid receptors within the antinociceptive brainstem circuits / A.E. Kalyuzhny --
In situ hybridization in neural tissues / H.B. Gutstein --
Quantitative single-cell RT-PCR for opioid receptors and housekeeping genes / S.C. Silbert --
Gene arrays and proteomics. A primer / L. Mouledous and H.B. Gutstein --
Opioid receptor oligomerization. Detection and functional characterization of interacting receptors / I. Gomes, J. Filipovska and L.A. Devi --
Recombinant opioid receptors. Structure-function relationship / J. Filipovska [and others] --
Receptor knock-out and gene targeting. Generation of knock-out mice / I. Sora, K. Ikeda and Y. Mishina --
Pharmacological interventions at the spinal cord. Intrathecal injections / D.A. Robinson and M. Zhuo --
Opioid tolerance in adult and neonatal rats / Z.Z. Pan --
Animal models of neuropathic pain / W.J. Martin, L.S. Stewart and J.W. Tarpley --
Place conditioning to study drug reward and aversion / W.A. Carlezon, Jr. --
Opiate self-administration / Z.X. Xi and E.A. Stein --
Acute, chronic, and cancer pain. Clinical management / A.W. Burton --
Clinical treatment of opioid addiction and dependence / W. Ling, R.A. Rawson and M. Compton.
Series Title: Methods in molecular medicine, 84.
Responsibility: edited by Zhizhong Z. Pan.
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Abstract:

Opioid research is one of the multidisciplinary research areas that involve advanced techniques ranging from molecular genetics to neuropharmacology, and from behavioral neuroscience to clinical  Read more...

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