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Optical tweezers : methods and protocols

Author: Arne Gennerich
Publisher: New York, NY : Humana Press, [2017] ©2017
Series: Methods in molecular biology (Clifton, N.J.), v. 1486.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: (OCoLC)950953638
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Arne Gennerich
ISBN: 9781493964215 1493964216
OCLC Number: 964656636
Notes: Includes bibliographical references and index.
Description: 1 online resource (xii, 555 pages) : illustrations (some color).
Contents: Introduction to optical tweezers --
Exact theory of optical tweezers and its application to absolute calibration --
Beyond the hookean spring model: Direct measurement of optical forces through light momentum changes --
A surface-coupled optical trap with 1-bp precision via active stabilization --
Implementation and tuning of an optical tweezers force-clamp feedback system --
Custom-made microspheres for optical tweezers --
Optical torque wrench design and calibration --
High-resolution "fleezers": Dual-trap optical tweezers combined with single-molecule fluorescence detection --
Versatile quadruple-trap optical tweezers for dual DNA experiments --
Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics --
Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement --
Mechanically watching the clpXP proteolytic machinery --
Deciphering the molecular mechanism of the bacteriophage phi29 DNA packaging motor --
Single-molecule protein folding experiments using high-precision optical tweezers --
Observing single RNA polymerase molecules down to base-pair resolution --
Optical tweezers-based measurements of forces and dynamics at microtubule ends --
Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips --
Measurement of force-dependent release rates of cytoskeletal motors --
Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers --
Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap --
Measuring molecular forces using calibrated optical tweezers in living cells.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1486.
Responsibility: edited by Arne Gennerich.

Abstract:

The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and  Read more...

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