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Overcoming molecular sample processing limitations : RNA/DNA extractions strategies

Author: James A Higgins
Publisher: Alexandria, VA : Water Environment Research Foundation, 2002.
Series: WERF Report Series.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

Overcoming Molecular Sample Processing Limitations: (a) New Platform Technologies, (b) RNA and DNA Extraction Strategies, and (c) Fiber-Optic Biosensors

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Genre/Form: Electronic books
Additional Physical Format: Print version:
Higgins, James A.
Overcoming molecular sample processing limitations.
(DLC) 2002107883
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: James A Higgins
ISBN: 9781780403151 1780403151
OCLC Number: 872681094
Notes: "00-HHE-2A."
"Pathogens and indicators"--Cover.
Description: 1 online resource (157 pages) : illustrations.
Contents: ""Cover""; ""Overcoming Molecular Sample Processing Limitations: RNA/DNA Extractions Strategies""; ""Preface""; ""Copyright""; ""Acknowledgments""; ""Abstract and Benefits""; ""Table of Contents""; ""List of Tables""; ""List of Figures""; ""List of Acronyms""; ""Executive Summary""; ""Chapter 1.0: Introduction and Methods""; ""1.1 Extraction of DNA from Bacteria""; ""1.2 Extraction of DNA from C. parvum Oocysts""; ""1.3 Extraction of DNA from Giardia lamblia Cysts""; ""1.4 Recovery of C. parvum Oocysts and G. lamblia Cysts by Continuous Flow Centrifugation (CFC)"" ""1.5 Detection of Oocysts and Cysts Using PCR""""1.6 Evaluation of Different PCR Primers for Detection of 10 or Fewer Oocysts""; ""1.7 Culture of MDBK Cells, RNA Extraction and Detection of (BEV) RNA""; ""1.8 Extraction of of Neucleic Acids Using the ABI 6100 Nucleic Acid Prepstation""; ""Chapter 2.0: Results""; ""2.1 Rapid Extraction of DNA from Bacterial Cultures""; ""2.2 Extraction of DNA from PurifiedC parvum Oocysts and G. lamblia Cysts""; ""2.3 Recovery of C parvum Oocysts and G. lamblia Cysts from Spiked Source Water Samples Using CFC"" ""2.4 Recovery of RNA from Cultured Cells and BEV""""2.5 Evaluation of Different PCR Primers for Detection of 10 or Fewer Oocysts""; ""Chapter 3.0: Discussion""; ""3.1 Extraction of DNA from Bacteria""; ""3.2 Extraction of DNA from C. parvum Oocysts and G. lamblia Cysts""; ""3.3 CFC for Recovery of Oocysts and Cysts from Spiked Source Water Samples""; ""3.4 Evaluation of Different PCR Primers for Detection of 10 or Fewer Oocysts""; ""3.5 Evaluation of Different RNA Extraction Methods""; ""3.6 Conclusion""; ""References "" ""Overcoming Molecular Sample Processing Limitations: Quantitative PCR""""Copyright""; ""Acknowledgments""; ""Abstract and Benefits""; ""Table of Contents""; ""List of Tables""; ""List of Figures""; ""List of Acronyms""; ""Executive Summary""; ""Chapter 1.0: Introduction""; ""1.1 Background""; ""1.2 Project Objectives""; ""Chapter 2.0: Research Approach""; ""2.1 Purification of Enteroviruses and Cryptosporidium""; ""2.1.1 Host Cell Capture of Potentially Infectious Enteroviruses""; ""2.1.2 Immunomagnetic Separation (IMS) of Cryptosporidium"" ""2.2 Quantitative Sequence Detection (QSD) Quantitative PCR""""2.2.1 Primers and Fluorogenic Probe for Cryptosporidium""; ""2.2.2 Primers and Fluorogenic Probe for Enteroviruses""; ""2.3 Analysis of Seeded and Unseeded Wastewater Samples""; ""2.3.1 Sample Processing for Cryptosporidium""; ""2.3.2 Sample Processing for Enteroviruses""; ""2.4 Genotyping of Cryptosporidium and Enterovirus Isolates""; ""Chapter 3.0: Methods Evaluation and Standards""; ""3.1 Host Cell Capture of Enterovirus""; ""3.1.1 Efficiency of Host Cell Capture""
Series Title: WERF Report Series.
Responsibility: by James A. Higgins, United States Department of Agriculture - Agricultural Research Service.

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