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P53 protocols

Author: Sumitra Deb; Swati Palit Deb
Publisher: New York : Humana Press, [2013] ©2013
Series: Methods in molecular biology (Clifton, N.J.), v. 234.
Edition/Format:   Print book : English : Second editionView all editions and formats
Database:WorldCat
Summary:

In its updated 2nd edition, this Methods in Molecular Biology(TM) book offers tools for identifying target genes, methods to determine stress response, autophagy or senescence induced by p53, cell  Read more...

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Genre/Form: Laboratory manuals
Document Type: Book
All Authors / Contributors: Sumitra Deb; Swati Palit Deb
ISBN: 9781627032353 1627032355
OCLC Number: 824171924
Description: x, 238 pages : illustrations (some color) ; 24 cm.
Contents: Detecting and quantifying p53 isoforms at mRNA level in cell lines and tissues / Marie P. Khoury [and others] --
Detecting p53 isoforms at protein level / Virginie Marcel [and others] --
Autophagy, senescence, and apoptosis / Rachel W. Goehe [and others] --
p53 and cell cycle effects after DNA damage / Emir Senturk and James J. Manfredi --
p53 ubiquitination and proteasomal degradation / Ian M. Love, Dingding Shi, and Steven R. Grossman --
Identification of p53 in mitochondria / Angelina V. Vaseva and Ute M. Moll --
Identification of novel mutant p53 interacting proteins by proteomic analysis / Sumitra Deb and Paul R. Graves --
Identification of small molecules affecting p53-MDM2/MDMX interaction by fluorescence polarization / Qi Zhang and Hua Lu --
Determine the effect of p53 on chemosensitivity / Emir Senturk and James J. Manfredi --
Measurement of chemosensitivity and growth rate in p53 expressing cells / Mahesh Ramamoorthy [and others] --
Mutant p53 in cell adhesion and motility / W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb --
Use of the DNA fiber spreading technique to detect the effects of mutant p53 on DNA replication / Rebecca A. Frum, Sumitra Deb, and Swati Palit Deb --
Generation of p53-deficient induced pluripotent stem cells from mouse embryo fibroblasts / Evguenia M. Alexandrova and Ute M. Moll --
p53 actions on microRNA expression and maturation pathway / Hiroshi I. Suzuki and Kohei Miyazono --
Isolation and characterization of murine multipotent lung stem cells / Venkat S. Gadepalli, Catherine Vaughan, and Raj R. Rao --
Generation of p53 knock-down cell lines / Catherine Vaughan, Swati Palit Deb, and Sumitra Deb --
ChIP for identification of p53 responsive DNA promoters / Jun-Ming Liao and Hua Lu --
ChIP-on-chip to identify mutant p53 targets / Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino --
ChIP sequencing to identify p53 targets / Catherine Vaughan, Brad Windle, and Sumitra Deb.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 234.
Responsibility: edited by Sumitra Deb, Department of Biochemistry, Massey Cancer Center, Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA, Swati Palit Deb, Department of Biochemistry, Massey Cancer Center, Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA.
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