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PCR protocols : current methods and applications

Author: Bruce Alan White
Publisher: Totowa, N.J. : Humana Press, ©1993.
Series: Methods in molecular biology (Clifton, N.J.), v. 15.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications.

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Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Bruce Alan White
ISBN: 0896032442 9780896032446
OCLC Number: 26762488
Description: xiv, 392 pages : illustrations ; 23 cm.
Contents: Polymerase chain reaction : basic protocols / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White --
Selection of primers for polymerase chain reaction / Wojciech Rychlik --
Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer --
Use of arithmetic polymerase chain reaction for synthesis of single-stranded probes for S1 nuclease assays / Puja Agarwal, Bruce A. White --
Nonradioactive labeling of polymerase chain reaction products / Udo Reischl, Rüdiger Rüger, Christoph Kessler --
Quantitation and purification of polymerase chain reaction products by high-performance liquid chromatography / Elena D. Katz --
Use of polymerase chain reaction for screening transgenic mice / Shizhong Chen, Glen A. Evans --
Polymerase chain reaction analysis of DNA from paraffin-embedded tissue / Matthias Volkenandt, Adam P. Dicker, Renato Fanin, Debabrata Banerjee, Anthony Albino, Joseph R. Bertino --
The use of polymerase chain reaction for chromosome assignment / Michael Jaye, Craig A. Dionne --
Mapping MHC class II genes and disease-susceptibility : use of polymerase chain reaction and dot hybridization for human leukocyte antigen allele typing / Stephen C. Bain, John A. Todd --
The use of the polymerase chain reaction and the detection of amplified products / Robert C. Allen, Bruce Budowle --
Determination of loss of heterozygosity using polymerase chain reaction / Stephen J. Meltzer --
Direct sequencing of polymerase chain reaction products / Stephen J. Meltzer --
Manual and automated direct sequencing of product generated by the polymerase chain reaction / Adam P. Dicker, Matthias Volkenandt, Joseph R. Bertino --
Genomic footprinting by ligation mediated polymerase chain reaction / Gerd P. Pfeifer, Arthur D. Riggs --
RNA template-specific polymerase chain reaction (RS-PCR) : a modification of RNA-PCR that dramatically reduces the frequency of false positives / Alan R. Shuldiner, Riccardo Perfetti, Jesse Roth --
Quantitative measurement of relative gene expression in human tumors / Tetsuro Horikoshi, Kathleen Danenberg, Matthias Volkenandt, Thomas Stadlbauer, Peter V. Danenberg --
Identification of alternatively spliced mRNAs and localization of 5' ends by polymerase chain reaction amplification / Cheng-Ming Chiang, Louise T. Chow, Thomas R. Broker --
Utilization of polymerase chain reaction for clonal analysis of gene expression / Alice L. Witsell, Lawrence B. Schook --
Sequencing DNA amplified directly from a bacterial colony / Martin A. Hofmann, David A. Brian --
Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem --
Molecular cloning of polymerase chain reaction fragments with cohesive ends / Beverly C. Delidow --
Rapid (ligase-free) subcloning of polymerase chain reaction products / Alan R. Shuldiner, Keith Tanner --
Use of polymerase chain reaction for making recombinant constructs / Douglas H. Jones, Stanley C. Winistorfer --
In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton --
Use of polymerase chain reaction for the rapid construction of synthetic genes / Patrick J. Dillon, Craig A. Rosen --
Recombinant circle polymerase chain reaction for site-directed mutagenesis / Douglas H. Jones --
Site-directed mutagenesis by double polymerase chain reaction : megaprimer method / Sailen Barik --
Generation of a polymerase chain reaction renewable source of subtractive cDNA / W. Michael Kuehl, James Battey --
PCR-based full-length cDNA cloning utilizing the universal-adaptor/specific DOS primer-pair strategy / David L. Cooper, Narayana R. Isola --
Use of degenerate oligonucleotide primers and the polymerase chain reaction to clone gene family members / Gregory M. Preston --
Single specific primer-polymerase chain reaction (SSP-PCR) and genome walking / Venkatakrishna Shyamala, Giovanna Ferro-Luzzi Ames --
cDNA cloning by inverse polymerase chain reaction / Sheng-He Huang, Chun-Hua Wu, Bing Cai, John Holcenberg --
Amplification of gene ends from gene libraries by polymerase chain reaction with single-sided specificity / Sheng-He Huang, Ambrose Y. Jong, Wu Yang, John Holcenberg --
Anchoring a defined sequence to the 55' ends of mRNAs : the bolt to clone rare full length mRNAs and generate cDNA libraries from a few cells / Jean Baptiste Dumas Milne Edwards, Jacques Delort, Jacques Mallet.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 15.
Responsibility: edited by Bruce A. White.
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...affords many new insights and contributions of the first order...an excellent book of the highest interest. - Cellular and Molecular Biology

 
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