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PCR sequencing protocols

Author: Ralph Rapley
Publisher: Totowa, N.J. : Humana Press, ©1996.
Series: Methods in molecular biology (Clifton, N.J.), v. 65.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA  Read more...

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Genre/Form: Aufsatzsammlung
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Online version:
PCR sequencing protocols.
Totowa, N.J. : Humana Press, ©1996
(OCoLC)606072463
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Ralph Rapley
ISBN: 0896033449 9780896033443
OCLC Number: 35262236
Description: xi, 221 pages : illustrations ; 23 cm.
Contents: Preparation and analysis of DNA sequencing gels --
Purification of PCR products from agarose gels for direct sequencing --
Enzymatic fluorescence and biotin labeling of primers for PCR sequencing --
Direct sequencing of double-stranded PCR products with the sequenase kit and [alpha-³⁵S] dATP --
Direct sequencing by thermal asymmetric PCR --
Rapid sequencing of cDNA clones : direct sequencing using sequential linear/asymmetric PCR --
Direct sequencing of PCR products using chemiluninescent detection --
Direct DNA sequencing of PCR products using magnetic beads --
Affinity capture and solid-phase sequencing of biotinylated PCR products --
Analysis of nucleotide sequence variations by solid-phase minisequencing --
Nonradioactive PCR sequencing using digoxigenin --
Silver Sequencing nonradioactive cycle sequencing of dsDNA --
Direct sequencing of PCR products with DNA-binding proteins --
PCR sequencing with the aid of detergents --
Direct sequencing with highly degenerate and inosine-containing primers --
Determination of unknown genomic sequences without cloning --
DNA sequencing by the chemical method --
Direct PCR sequencing with denaturants (formamide) --
Efficient PCR production of single-stranded DNA sequencing templates --
Preparation and direct automated cycle sequencing of PCR products --
Solid-phase automated sequencing of PCR-ampilified genomic DNA --
Cloning PCR products for sequencing in M13 vectors --
Sequencing PCR products cloned into M13 vectors --
Genomic ampilificatin with transcript sequencing (GAWTS) --
DNA rescue by the vectorette method --
Sequencing of (dA:dT) cloned mixed PCR products from microbial populations.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 65.
Responsibility: edited by Ralph Rapley.
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...this is a good collection of well presented PCR protocols that should find its way into many laboratories...if you are planning on performing a large amount of PCR sequencing, or have been having Read more...

 
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