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PCR technology : principles and applications for DNA amplification

Author: Henry A Erlich
Publisher: Macmillan, 1989.
Series: Breakthroughs in molecular biology.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all  Read more...

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Document Type: Book
All Authors / Contributors: Henry A Erlich
ISBN: 0333489489 9780333489482 093585956X 9780935859560
OCLC Number: 19267540
Description: ix, 246 pages
Contents: Part 1 Introduction to the PCR: history of PCR development; description of the PCR method - basic principles and properties of the PCR; thermostable DNA polymerases; automation; sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR; preparation of specific DNA fragments using PCR; detection of gene expression; genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens; diagnosis of monogenic genetic diseases; HLA DNA typing and disease susceptibility; forensic applications; detection of activated oncogenes; future developments and applications.
Series Title: Breakthroughs in molecular biology.
Responsibility: Henry A. Erlich editor.

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