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Perspectives on Solid State NMR in Biology

Author: S R Kiihne; Huub J M Groot
Publisher: Dordrecht : Springer Netherlands, 2001.
Series: Focus on structural biology, 1.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Solid state NMR is rapidly emerging as a universally applicable method for the characterization of ordered structures that cannot be studied with solution methods or diffraction techniques. This proceedings -; from a recent international workshop - captures an image of the latest developments and future directions for solid state NMR in biological research, particularly on membrane proteins. Detailed information on  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: S R Kiihne; Huub J M Groot
ISBN: 9789401725798 9401725799
OCLC Number: 851368750
Description: 1 online resource (xi, 233 pages).
Contents: Section I: Advances in MAS Techniques for Non-Aligned Systems --
Using symmetry to design pulse sequences in solid-state NMR --
Accurate 13C-15N Distance Measurements in Uniformly 13C,15N-Labeled Peptides --
Selectivity of Double-Quantum Filtered Rotational-Resonance Experiments on Larger-than-Two-Spin Systems --
Multiple-quantum spectroscopy of fully labeled polypeptides under MAS: A statistical and experimental analysis --
Section II: Advances in Techniques for Aligned Systems --
2H, 15N and 31P solid-state NMR spectroscopy of polypeptides reconstituted into oriented phospholipid membranes --
From Topology to High Resolution Membrane Protein Structures --
Toward dipolar recoupling in macroscopically ordered samples of membrane proteins rotating at the magic angle --
Solid state 19F-NMR of biomembranes --
Section III: Important Technologies --
Numerical simulations for experiment design and extraction of structural parameters in biological solid-state NMR spectroscopy --
An ab-initio molecular dynamics modeling of the primary photochemical event in vision --
Refolded G protein-coupled receptors from E. coli inclusion bodies --
Semliki Forest virus vectors: versatile tools for efficient large-scale expression of membrane receptors --
G protein-coupled receptor expression in Halobacterium salinarum --
Magnetic resonance microscopy for studying the development of chicken and mouse embryos --
Section IV: Applications in Membrane Proteins and Peptides --
MAS NMR on a uniformly [13C 15N] labeled LH2 light-harvesting complex from Rhodopseudomonas acidophila 10050 at ultra-high magnetic fields --
Determination of Torsion Angles in Membrane Proteins --
Characterization and assignment of uniformly labeled NT(8-13) at the agonist binding site of the G-protein coupled neurotensin receptor --
Structural insight into the interaction of amyloid-? peptide with biological membranes by solid state NMR. --
Photochemically induced dynamic nuclear polarization in bacterial photosynthetic reaction centres observed by 13C solid-state NMR.
Series Title: Focus on structural biology, 1.
Responsibility: edited by Suzanne R. Kiihne, Huub J.M. Groot.

Abstract:

Solid state NMR is rapidly emerging as a universally applicable method for the characterization of ordered structures that cannot be studied with solution methods or diffraction techniques.  Read more...

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