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Phage display of peptides and proteins : a laboratory manual

Author: Brian K Kay; Jill Winter; John McCafferty, Dr.
Publisher: San Diego : Academic Press, 1996.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Phage display of peptides and proteins.
San Diego : Academic Press, 1996
(DLC) 96013576
(OCoLC)34409484
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Brian K Kay; Jill Winter; John McCafferty, Dr.
ISBN: 9780124023802 0124023800 9780080538709 0080538703
OCLC Number: 162129004
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xxii, 344 pages) : illustrations (some color)
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Foreword. Biology of the Filamentous Bacteriophage. Principles and Applications of Phage Display. Vectros for Phage Display. Construction of Random Peptide Libraries in Bacteriophage. Construction and Screening of Antibody Display Libraries. Phagemeid-Displayed Peptide Libraries. Phage Libraries Displaying Random Peptides. Derived Form a Target Sequence. Display and Selection of Proteins on Genetic Packages. A Cdna Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products By Protein/Ligand Interactions Made Possible By Linkage of Recogntion and Replication Functions. Multicombinational Libraries and Combinational Infection: Practical Considerations for Vector Design. Use of Filamentous Virus Particles as Immunogens. Screening Phage Displayed Random Peptide Libraries. Phage Display Factors Affecting Planning Efficieny. Preparation of Second Generation Phage Libraries. Nonradioactive Sequencing of Random Peptide Recombinant Phage. Measurement of Peptide Binding Affinities Using Fluorescence Polarization.
Responsibility: edited by Brian K. Kay, Jill Winter, John McCafferty.
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Abstract:

This volume provides a review of the literature and outlines the strategies for maximizing the successful application of phage display technology to research. It contains the up-to-date protocols for  Read more...

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